The iNOS Antibody (CAB0312) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, specifically targets human NOS2 and has been validated for use in Western blot applications. By binding to the NOS2 protein, this antibody allows for the detection and analysis of NOS2 in various cell types, making it ideal for studies in immunology, infectious diseases, and inflammatory conditions.NOS2, also known as inducible nitric oxide synthase, plays a key role in the production of nitric oxide, a signaling molecule involved in immune defense and inflammation.
This antibody is validated for use in IF/ICC, ELISA applications and has demonstrated reactivity against Human, Rat samples.
Product Name:
iNOS Antibody
SKU:
CAB0312
Size:
20μL, 100μL
Reactivity:
Human, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
NOS, INOS, NOS2A, HEP-NOS, iNOS
Cellular Localization:
Cortical Cytoskeleton, Cytoplasm, Cytosol, Nucleoplasm, Nucleus, Perinuclear Region Of Cytoplasm, Peroxisomal Matrix, Peroxisome, Plasma Membrane.
Calculated MW:
131kDa
Observed MW:
Refertofigures
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.
Purification Method
Affinity purification
Gene ID
4843
RRID
AB_2757120
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from Caco-2 cells using iNOS Rabbit pAb (CAB0312) at 1:2000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30 s.
