Anti-NR2E1 Antibody (CAB13361)

Antibody Name:	Anti-NR2E1 Antibody
Antibody SKU:	CAB13361
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 106-385 of human NR2E1 (NP_003260.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human
Positive Samples:	HL-60, HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 106-385 of human NR2E1 (NP_003260.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	YFRG HKEE NGAA AHFP SAAL PAPA FFTA VTQL EPHG LELA AVST TPER QTLV SLAQ PTPK YPHE VNGT PMYL YEVA TESV CESA ARLL FMSI KWAK SVPA FSTL SLQD QLML LEDA WREL FVLG IAQW AIPV DANT LLAV SGMN GDNT DSQK LNKI ISEI QALQ EVVA RFRQ LRLD ATEF ACLK CIVT FKAV PTHS GSEL RSFR NAAA IAAL QDEA QLTL NSYI HTRY PTQP CRFG KLLL LLPA LRSI SPST IEEV FFKK TIGN VPIT RLLS DMYK SSDI
Gene ID:	7101
Uniprot:	Q9Y466
Cellular Location:	Nucleus
Calculated MW:	42kDa/46kDa
Observed MW:	43kDa

Synonyms:	NR2E1, TLL, TLX, XTLL
Background:	The protein encoded by this gene is an orphan receptor involved in retinal development. The encoded protein also regulates adult neural stem cell proliferation and may be involved in control of aggressive behavior. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	NR2E1: Orphan receptor that binds DNA as a monomer to hormone response elements (HRE) containing an extended core motif half- site sequence 5'-AAGGTCA-3' in which the 5' flanking nucleotides participate in determining receptor specificity. May be required to pattern anterior brain differentiation. Involved in the regulation of retinal development and essential for vision. During retinogenesis, regulates PTEN-Cyclin D expression via binding to the promoter region of PTEN and suppressing its activity. May be involved in retinoic acic receptor (RAR) regulation in retinal cells. Belongs to the nuclear hormone receptor family. NR2 subfamily.
UniProt Protein Details:	Protein type:DNA-binding; Nuclear receptor Chromosomal Location of Human Ortholog: 6q21 Cellular Component: nucleoplasm Molecular Function:zinc ion binding; sequence-specific DNA binding; histone deacetylase binding; steroid hormone receptor activity Biological Process: extracellular matrix organization and biogenesis; behavioral fear response; somatic stem cell maintenance; regulation of timing of neuron differentiation; regulation of dendrite morphogenesis; amygdala development; negative regulation of transcription from RNA polymerase II promoter; cerebral cortex neuron differentiation; visual perception; layer formation in the cerebral cortex; transcription initiation from RNA polymerase II promoter; nervous system development; cell fate commitment; dentate gyrus development; generation of neurons in the forebrain; olfactory bulb development; positive regulation of cell cycle; social behavior; positive regulation of angiogenesis; negative regulation of neuron differentiation; anterior commissure morphogenesis; retina development in camera-type eye; negative regulation of astrocyte differentiation; gene expression; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; negative regulation of apoptosis
NCBI Summary:	The protein encoded by this gene is an orphan receptor involved in retinal development. The encoded protein also regulates adult neural stem cell proliferation and may be involved in control of aggressive behavior. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015]
UniProt Code:	Q9Y466
NCBI GenInfo Identifier:	9910804
NCBI Gene ID:	7101
NCBI Accession:	Q9Y466.1
UniProt Secondary Accession:	Q9Y466,Q6ZMP8,
UniProt Related Accession:	Q9Y466
Molecular Weight:	385
NCBI Full Name:	Nuclear receptor subfamily 2 group E member 1
NCBI Synonym Full Names:	nuclear receptor subfamily 2, group E, member 1
NCBI Official Symbol:	NR2E1
NCBI Official Synonym Symbols:	TLL; TLX; XTLL
NCBI Protein Information:	nuclear receptor subfamily 2 group E member 1; tailless homolog; nuclear receptor TLX; tailes-related receptor; protein tailless homolog
UniProt Protein Name:	Nuclear receptor subfamily 2 group E member 1
UniProt Synonym Protein Names:	Nuclear receptor TLX; Protein tailless homolog; Tll; hTll
Protein Family:	Nuclear receptor subfamily
UniProt Gene Name:	NR2E1
UniProt Entry Name:	NR2E1_HUMAN

	Western blot analysis of extracts of various cell lines, using NR2E1 antibody (CAB13361) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of A549 cells using NR2E1 antibody (CAB13361).

Antibodies

Anti-NR2E1 Antibody (CAB7455)

Anti-NR2E1 Antibody (CAB8768)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)