Anti-NTF4 Antibody (CAB0912)

Antibody Name:	Anti-NTF4 Antibody
Antibody SKU:	CAB0912
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 25-210 of human NTF4 (NP_006170.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human
Positive Samples:	HT-1080

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 25-210 of human NTF4 (NP_006170.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QPPP STLP PFLA PEWD LLSP RVVL SRGA PAGP PLLF LLEA GAFR ESAG APAN RSRR GVSE TAPA SRRG ELAV CDAV SGWV TDRR TAVD LRGR EVEV LGEV PAAG GSPL RQYF FETR CKAD NAEE GGPG AGGG GCRG VDRR HWVS ECKA KQSY VRAL TADA QGRV GWRW IRID TACV CTLL SRTG RA
Gene ID:	4909
Uniprot:	P34130
Cellular Location:	Secreted
Calculated MW:	22kDa
Observed MW:	35kDa

Synonyms:

NTF4, GLC10, GLC1O, NT-4, NT-4/5, NT-5, NT4, NT5, NTF5

Background:

This gene is a member of a family of neurotrophic factors, neurotrophins, that control survival and differentiation of mammalian neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. While knock-outs of other neurotrophins including nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 prove lethal during early postnatal development, NTF5-deficient mice only show minor cellular deficits and develop normally to adulthood.

UniProt Protein Function:	NT-4/5: Target-derived survival factor for peripheral sensory sympathetic neurons. Defects in NTF4 may be associated with susceptibility to primary open angle glaucoma type 1O (GLC1O). A form of primary open angle glaucoma (POAG). POAG is characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place. Belongs to the NGF-beta family.
UniProt Protein Details:	Protein type:Cytokine; Secreted; Cell development/differentiation; Secreted, signal peptide Chromosomal Location of Human Ortholog: 19q13.3 Cellular Component: endoplasmic reticulum lumen; cytoplasmic membrane-bound vesicle; extracellular region Molecular Function:neurotrophin p75 receptor binding; growth factor activity Biological Process: mechanoreceptor differentiation; epidermis development; cell-cell signaling; long-term memory; regulation of neuron differentiation; adult locomotory behavior; sensory organ boundary specification; ganglion mother cell fate determination; negative regulation of neuron apoptosis; transmembrane receptor protein tyrosine kinase signaling pathway; neurite morphogenesis Disease: Glaucoma 1, Open Angle, O
NCBI Summary:	This gene is a member of a family of neurotrophic factors, neurotrophins, that control survival and differentiation of mammalian neurons. The expression of this gene is ubiquitous and less influenced by environmental signals. While knock-outs of other neurotrophins including nerve growth factor, brain-derived neurotrophic factor, and neurotrophin 3 prove lethal during early postnatal development, NTF5-deficient mice only show minor cellular deficits and develop normally to adulthood. [provided by RefSeq, Jul 2008]
UniProt Code:	P34130
NCBI GenInfo Identifier:	462741
NCBI Gene ID:	4909
NCBI Accession:	P34130.1
UniProt Secondary Accession:	P34130,Q6FH56,
UniProt Related Accession:	P34130,AAB20549
Molecular Weight:	210
NCBI Full Name:	Neurotrophin-4
NCBI Synonym Full Names:	neurotrophin 4
NCBI Official Symbol:	NTF4
NCBI Official Synonym Symbols:	NT4; NT5; NT-4; NT-5; NTF5; GLC10; GLC1O; NT-4/5
NCBI Protein Information:	neurotrophin-4; neurotrophin-5; neutrophic factor 4; neurotrophic factor 4; neurotrophic factor 5; neurotrophin 5 (neurotrophin 4/5)
UniProt Protein Name:	Neurotrophin-4
UniProt Synonym Protein Names:	Neurotrophin-5; NT-5; Neutrophic factor 4
Protein Family:	Neurotrophin
UniProt Gene Name:	NTF4
UniProt Entry Name:	NTF4_HUMAN

	Western blot analysis of extracts of HT-1080 cells, using NTF4 antibody (CAB0912) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded human colon using NTF4 antibody (CAB0912) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human prostate using NTF4 antibody (CAB0912) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-NTF4 Antibody (CAB1081)	Human NT-4 (Neurotrophin 4) CLIA Kit (HUES01046)
Anti-NTF4 Antibody (CAB3180)	Human Neurotrophin-4 (NTF4) ELISA Kit
NTF4 Antibody (PACO04465)
NTF4 Antibody (PACO10943)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)