Anti-PCBP2 Antibody (CAB2531)

Antibody Name:	Anti-PCBP2 Antibody
Antibody SKU:	CAB2531
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human PCBP2 (NP_001122383.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW620, MCF7, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human PCBP2 (NP_001122383.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDTG VIEG GLNV TLTI RLLM HGKE VGSI IGKK GESV KKMR EESG ARIN ISEG NCPE RIIT LAGP TNAI FKAF AMII DKLE EDIS SSMT NSTA ASRP PVTL RLVV PASQ CGSL IGKG GCKI KEIR ESTG AQ
Gene ID:	5094
Uniprot:	Q15366
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	33kDa/34kDa/35kDa/38kDa
Observed MW:	39kDa

Synonyms:

PCBP2, HNRNPE2, HNRPE2, hnRNP-E2

Background:

The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. Thsi gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	hnRNP E2: Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Negatively regulates cellular antiviral responses mediated by MAVS signaling. It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitinationa and degradation. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:RNA splicing; RNA-binding Chromosomal Location of Human Ortholog: 12q13.13 Cellular Component: nucleoplasm; focal adhesion; membrane; cytoplasm; cytosol; ribonucleoprotein complex; nucleus Molecular Function:protein binding; enzyme binding; DNA binding; RNA binding; ubiquitin protein ligase binding Biological Process: nuclear mRNA splicing, via spliceosome; proteasomal ubiquitin-dependent protein catabolic process; RNA splicing; innate immune response; gene expression; negative regulation of defense response to virus; defense response to virus; negative regulation of interferon type I production; mRNA metabolic process
NCBI Summary:	The protein encoded by this gene appears to be multifunctional. Along with PCBP-1 and hnRNPK, it is one of the major cellular poly(rC)-binding proteins. The encoded protein contains three K-homologous (KH) domains which may be involved in RNA binding. Together with PCBP-1, this protein also functions as a translational coactivator of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES, promoting poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1, an intronless gene with functions similar to that of PCBP2. This gene and PCBP-1 have paralogous genes (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. This gene also has two processed pseudogenes (PCBP2P1 and PCBP2P2). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2018]
UniProt Code:	Q15366
NCBI GenInfo Identifier:	6707736
NCBI Gene ID:	5094
NCBI Accession:	Q15366.1
UniProt Related Accession:	Q15366
Molecular Weight:
NCBI Full Name:	Poly(rC)-binding protein 2
NCBI Synonym Full Names:	poly(rC) binding protein 2
NCBI Official Symbol:	PCBP2
NCBI Official Synonym Symbols:	HNRPE2; HNRNPE2; hnRNP-E2
NCBI Protein Information:	poly(rC)-binding protein 2
UniProt Protein Name:	Poly(rC)-binding protein 2
UniProt Synonym Protein Names:	Alpha-CP2; Heterogeneous nuclear ribonucleoprotein E2; hnRNP E2
Protein Family:	Poly(rC)-binding protein
UniProt Gene Name:	PCBP2
UniProt Entry Name:	PCBP2_HUMAN

	Western blot analysis of extracts of various cell lines, using PCBP2 antibody (CAB2531) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded mouse brain using PCBP2 Rabbit pAb (CAB2531) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using PCBP2 Rabbit pAb (CAB2531) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of NIH-3T3 cells using PCBP2 Rabbit pAb (CAB2531) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using PCBP2 Rabbit pAb (CAB2531) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

PCBP2 Antibody (PACO11090)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)