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Anti-PCK2 Antibody (CAB8446)

SKU:
CAB8446
Product Type:
Antibody
Applications:
WB
IHC
IP
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism
  • Metabolism Antibodies 3 Anti-PCK2 Antibody CAB8446
  • Metabolism Antibodies 3 Anti-PCK2 Antibody CAB8446
  • Metabolism Antibodies 3 Anti-PCK2 Antibody CAB8446
  • Metabolism Antibodies 3 Anti-PCK2 Antibody CAB8446
  • Metabolism Antibodies 3 Anti-PCK2 Antibody CAB8446
€139 - €419
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Description

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Antibody Name:Anti-PCK2 Antibody
Antibody SKU:CAB8446
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PCK2 (NP_004554.2).
Application:WB IHC IF IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 IP 1:20 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:SH-SY5Y, LO2, HepG2, Mouse testis, Mouse brain, Mouse kidney, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PCK2 (NP_004554.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAAL YRPG LRLN WHGL SPLG WPSC RSIQ TLRV LSGD LGQL PTGI RDFV EHSA RLCQ PEGI HICD GTEA ENTA TLTL LEQQ GLIR KLPK YNNC WLAR TDPK DVAR VESK TVIV TPSQ RDTV QLPP GGAR GQLG NWMS PADF QRAV DERF PGCM QGRT MYVL PFSM GPVG SPLS RIGV QLTD SAYV VASM RIMT RLGT PVLQ ALGD GDFV KCLH SVGQ PLTG QGEP VSQW PCNP EKTL IGHV PDQR EIIS FGSG YGGN SLLG KKCF ALRI ASRL ARDE GWLA
Gene ID:5106
Uniprot:Q16822
Cellular Location:Mitochondrion
Calculated MW:47kDa/70kDa
Observed MW:71kDa
Synonyms:PCK2, PEPCK, PEPCK-M, PEPCK2
Background:This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described.
UniProt Protein Function:PCK2: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. Monomer. Belongs to the phosphoenolpyruvate carboxykinase [GTP] family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 4.1.1.32; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other; Carbohydrate Metabolism - citrate (TCA) cycle; Mitochondrial; Lyase

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: mitochondrial matrix; mitochondrion

Molecular Function:phosphoenolpyruvate carboxykinase (GTP) activity; phosphoenolpyruvate carboxykinase activity; protein binding

Biological Process: gluconeogenesis

Disease: Phosphoenolpyruvate Carboxykinase Deficiency, Mitochondrial

NCBI Summary:This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2014]
UniProt Code:Q16822
NCBI GenInfo Identifier:290457671
NCBI Gene ID:5106
NCBI Accession:Q16822.3
UniProt Secondary Accession:Q16822,O43253, Q86U01, Q9BV62,
UniProt Related Accession:Q16822
Molecular Weight:47,564 Da
NCBI Full Name:Phosphoenolpyruvate carboxykinase
NCBI Synonym Full Names:phosphoenolpyruvate carboxykinase 2, mitochondrial
NCBI Official Symbol:PCK2  
NCBI Official Synonym Symbols:PEPCK; PEPCK2; PEPCK-M  
NCBI Protein Information:phosphoenolpyruvate carboxykinase [GTP], mitochondrial
UniProt Protein Name:Phosphoenolpyruvate carboxykinase [GTP], mitochondrial
Protein Family:Protein kinase
UniProt Gene Name:PCK2  
UniProt Entry Name:PCKGM_HUMAN
Anti-PCK2 Polyclonal Antibody (CAB8446)Western blot analysis of extracts of various cell lines, using PCK2 antibody (CAB8446) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 10s.
Anti-PCK2 Polyclonal Antibody (CAB8446)Immunohistochemistry of paraffin-embedded mouse kidney using PCK2 Rabbit pAb (CAB8446) at dilution of 1:100 (40x lens).
Anti-PCK2 Polyclonal Antibody (CAB8446)Immunohistochemistry of paraffin-embedded rat kidney using PCK2 Rabbit pAb (CAB8446) at dilution of 1:100 (40x lens).
Anti-PCK2 Polyclonal Antibody (CAB8446)Immunofluorescence analysis of NIH-3T3 cells using PCK2 Rabbit pAb (CAB8446) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Anti-PCK2 Polyclonal Antibody (CAB8446)Immunofluorescence analysis of U-2 OS cells using PCK2 Rabbit pAb (CAB8446) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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