The Proprotein Convertase 9(PCSK9) Monoclonal Antibody (CAB11532) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody was developed using advanced rabbit monoclonal technology, resulting in high specificity and sensitivity for detecting PCSK9 in human samples.PCSK9 has emerged as a key player in regulating cholesterol levels by targeting the LDL receptor for degradation, leading to increased LDL cholesterol levels in the bloodstream. Dysregulation of PCSK9 has been linked to a higher risk of cardiovascular diseases, making it a promising target for therapeutic interventions.With its superior performance in immunohistochemistry and ELISA applications, this PCSK9 antibody enables precise and reliable detection of PCSK9 protein expression in various tissues and cell types.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. The encoded protein undergoes an autocatalytic processing event with its prosegment in the ER and is constitutively secreted as an inactive protease into the extracellular matrix and trans-Golgi network. It is expressed in liver, intestine and kidney tissues and escorts specific receptors for lysosomal degradation. It plays a role in cholesterol and fatty acid metabolism. Mutations in this gene have been associated with autosomal dominant familial hypercholesterolemia. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
255738
RRID
AB_2861589
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HepG2 cells, using [KD Validated] Proprotein Convertase 9(PCSK9) Rabbit mAb (CAB11532) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of lysates from HepG2 cells, using [KD Validated] Proprotein Convertase 9(PCSK9) Rabbit mAb (CAB11532) at1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
