Anti-PGM1 Antibody (CAB13679)

Antibody Name:	Anti-PGM1 Antibody
Antibody SKU:	CAB13679
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PGM1 (NP_002624.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-251MG, HeLa, HepG2, 293T, Mouse liver, Mouse heart, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human PGM1 (NP_002624.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MVKI VTVK TQAY QDQK PGTS GLRK RVKV FQSS ANYA ENFI QSII STVE PAQR QEAT LVVG GDGR FYMK EAIQ LIAR IAAA NGIG RLVI GQNG ILST PAVS CIIR KIKA IGGI ILTA SHNP GGPN GDFG IKFN ISNG GPAP EAIT DKIF QISK TIEE YAVC PDLK VDLG VLGK QQFD LENK FKPF TVEI VDSV EAYA TMLR SIFD FSAL KELL SGPN RLKI RIDA MHGV VGPY VKKI LCEE LGAP ANSA VNCV PLED FGGH HPDP NLTY AADL VETM KSGE HDFG AAFD GDGD RNMI LGKH
Gene ID:	5236
Uniprot:	P36871
Cellular Location:	Cytoplasm
Calculated MW:	40kDa/61kDa/63kDa
Observed MW:	61kDa

Synonyms:

PGM1, CDG1T, GSD14

Background:

The protein encoded by this gene is an isozyme of phosphoglucomutase (PGM) and belongs to the phosphohexose mutase family. There are several PGM isozymes, which are encoded by different genes and catalyze the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, this PGM isozyme is predominant, representing about 90% of total PGM activity. In red cells, PGM2 is a major isozyme. This gene is highly polymorphic. Mutations in this gene cause glycogen storage disease type 14. Alternativley spliced transcript variants encoding different isoforms have been identified in this gene.

UniProt Protein Function:	This enzyme participates in both the breakdown and synthesis of glucose.
NCBI Summary:	The protein encoded by this gene is an isozyme of phosphoglucomutase (PGM) and belongs to the phosphohexose mutase family. There are several PGM isozymes, which are encoded by different genes and catalyze the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, this PGM isozyme is predominant, representing about 90% of total PGM activity. In red cells, PGM2 is a major isozyme. This gene is highly polymorphic. Mutations in this gene cause glycogen storage disease type 14. Alternativley spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Mar 2010]
UniProt Code:	P36871
NCBI GenInfo Identifier:	585670
NCBI Gene ID:	5236
NCBI Accession:	P36871.3
UniProt Secondary Accession:	P36871,Q16105, Q16106, Q5BKZ9, Q6NW22, Q86U74, Q96J40 Q9NTY4, B2R5N9, B4DPV0,
UniProt Related Accession:	P36871
Molecular Weight:	40,283 Da
NCBI Full Name:	Phosphoglucomutase-1
NCBI Synonym Full Names:	phosphoglucomutase 1
NCBI Official Symbol:	PGM1
NCBI Official Synonym Symbols:	CDG1T; GSD14
NCBI Protein Information:	phosphoglucomutase-1
UniProt Protein Name:	Phosphoglucomutase-1
UniProt Synonym Protein Names:	Glucose phosphomutase 1
Protein Family:	Phosphoglucomutase
UniProt Gene Name:	PGM1
UniProt Entry Name:	PGM1_HUMAN

Western blot analysis of extracts of various cell lines, using PGM1 antibody (CAB13679) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.

Antibodies	ELISA Kits
Anti-PGM1 Antibody (CAB6303)	Human PGM1 (Phosphoglucomutase-1) ELISA Kit (HUFI07254)
Anti-PGM1 Antibody (CAB19904)[KO Validated]	Human Phosphoglucomutase-1 (PGM1) ELISA Kit
Anti-PGM1 Antibody (CAB19905)[KO Validated]
PGM1 Antibody (PACO11199)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)