The Phospho-ATF2-T71 Monoclonal Antibody (CABP1051) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, is validated for use in various applications such as Western blotting, immunofluorescence, and immunohistochemistry.Phosphorylation of ATF2 at threonine 71 is known to regulate its transcriptional activity and is involved in signaling pathways related to stress responses, cell proliferation, and apoptosis. This antibody specifically recognizes the phosphorylated form of ATF2 at threonine 71, enabling precise detection and analysis of ATF2 activation in different cell types and experimental conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse samples.
Product Name:
Phospho-ATF2-T71 Monoclonal Antibody
SKU:
CABP1051
Size:
20μL, 100μL
Reactivity:
Mouse
Clone Number:
ARC1598
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
TPTP T
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene
Purification Method
Affinity purification
Gene ID
1386
RRID
AB_2863924
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-ATF2-T71 Rabbit mAb (CABP1051) at 1:1000 dilution. NIH/3T3 cells were treated with Anisomycin (25 μg/mL) at 37℃ for 30 minutes. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
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