The ELK1 Antibody (CAB0789) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, has high specificity for Elk1 and has been validated for use in Western blotting applications. By targeting Elk1, this antibody allows for the detection and analysis of Elk1 protein levels in various cell types, providing valuable insights for studies in molecular biology and cancer research.Elk1 plays a pivotal role in controlling gene expression in response to various cellular signals, making it a key player in processes such as cell proliferation, differentiation, and survival.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
ELK1 Antibody
SKU:
CAB0789
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ELK1
Positive Sample:
HeLa, Mouse placenta, Rat heart
Cellular Localization:
Nucleus.
Calculated MW:
45kDa
Observed MW:
62kDa
This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14.
Purification Method
Affinity purification
Gene ID
2002
RRID
AB_2757397
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using ELK1 Rabbit pAb (CAB0789) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
