| Western blot analysis of extracts of Jurkat cells, using Anti-Phospho-FoxO1-T24/FoxO3a-T32/FoxO4-T28 pAb (CABP0892) at 1:1000 dilution.Jurkat cells were treated by ATP(5 mM) at 30'C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
| Western blot analysis of extracts of NIH/3T3 cells, using Anti-Phospho-FoxO1-T24/FoxO3a-T32/FoxO4-T28 pAb (CABP0892) at 1:1000 dilution or FOXO1 antibody (CAB13862).NIH/3T3 cells were treated by ATP(5 mM) at 30'C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
| Immunohistochemistry of paraffin-embedded rat liver using Phospho-FoxO1-T24/FoxO3a-T32/FoxO4-T28 antibody (CABP0892) at dilution of 1:200 (40x lens). |
| Immunohistochemistry of paraffin-embedded human liver cancer using Phospho-FoxO1-T24/FoxO3a-T32/FoxO4-T28 antibody (CABP0892) at dilution of 1:200 (40x lens). |
| Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-FoxO1-T24/FoxO3a-T32/FoxO4-T28 antibody (CABP0892) at dilution of 1:200 (40x lens). |