The Phospho-FOXO4-S197 Antibody (CABP0177) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, specifically detects phosphorylation of FOXO4 at serine 197 in human samples, making it an ideal choice for Western blot applications.FOXO4 is a key player in the regulation of cell survival and proliferation, and its phosphorylation at serine 197 is known to impact its activity and subcellular localization. By targeting this specific phosphorylation site, researchers can gain insights into the signaling pathways that control FOXO4-mediated cellular functions.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Phospho-FOXO4-S197 Antibody
SKU:
CABP0177
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
AASM D
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:100 - 1:500
IF/ICC
1:100 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
AFX, AFX1, MLLT7, Phospho-FOXO4-S197
Positive Sample:
293F, Jurkat
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
54kDa
Observed MW:
65kDa
This gene encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving this gene on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein, located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method
Affinity purification
Gene ID
4303
RRID
AB_2771128
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using Phospho-FOXO4-S197 Rabbit pAb (CABP0177) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
Western blot analysis of various lysates, using Phospho-FOXO4-S197 Rabbit pAb (CABP0177) at 1:400 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
