The Phospho-MAP3K5-S1033 Antibody (CABP0058) is a high-quality antibody developed for reliable detection and analysis of target proteins. MAP3K5, also known as apoptosis signal-regulating kinase 1 (ASK1), is a key player in the MAPK signaling pathway, regulating diverse cellular processes such as apoptosis, inflammation, and stress responses.This antibody, produced in rabbits, exhibits high specificity and reactivity with human samples, making it ideal for Western blot applications. By targeting the phosphorylated form of MAP3K5, researchers can investigate the activation of MAPK signaling cascades and the downstream effects on cellular physiology and pathology.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-MAP3K5-S1033 Antibody
SKU:
CABP0058
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
PPSP E
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:2000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
ASK1, MEKK5, MAPKKK5, Phospho-MAP3K5-S1033
Positive Sample:
HeLa
Cellular Localization:
Cytoplasm, Endoplasmic Reticulum.
Calculated MW:
155kDa
Observed MW:
155kDa
Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK.
Purification Method
Affinity purification
Gene ID
4217
RRID
AB_2771298
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from A-431 and HeLa cells, using Phospho-MAP3K5-S1033 Rabbit pAb (CABP0058) at 1:1000 dilution. A431 cells were treated by PMA/TPA (200nM). HeLa cells were treated by 10% FBS or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA.
