Anti-MEF2C Antibody (CAB12385)

Antibody Name:	Anti-MEF2C Antibody
Antibody SKU:	CAB12385
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 170-380 of human MEF2C (NP_001180279.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 170-380 of human MEF2C (NP_001180279.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LPLA HPSL QRNS MSPG VTHR PPSA GNTG GLMG GDLT SGAG TSAG NGYG NPRN SPGL LVSP GNLN KNMQ AKSP PPMN LGMN NRKP DLRV LIPP GSKN TMPS VSED VDLL LNQR INNS QSAQ SLAT PVVS VATP TLPG QGMG GYPS AIST TYGT EYSL SSAD LSSL SGFN TASA LHLG SVTG WQQQ HLHN MPPS ALSQ LGAC TSTH LSQS SNL
Gene ID:	4208
Uniprot:	Q06413
Cellular Location:	Nucleus
Calculated MW:	44kDa/47kDa/50kDa/51kDa/52kDa
Observed MW:	45kDa

Synonyms:

MEF2C, C5DELq14.3, DEL5q14.3

Background:

This locus encodes a member of the MADS box transcription enhancer factor 2 (MEF2) family of proteins, which play a role in myogenesis. The encoded protein, MEF2 polypeptide C, has both trans-activating and DNA binding activities. This protein may play a role in maintaining the differentiated state of muscle cells. Mutations and deletions at this locus have been associated with severe mental retardation, stereotypic movements, epilepsy, and cerebral malformation. Alternatively spliced transcript variants have been described.

UniProt Protein Function:	MEF2C: transcription factor of the MADS family which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. May be involved in myogenesis, neurogenesis and in the development of cortical architecture. Three splice-variant isoforms have been described.
UniProt Protein Details:	Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 5q14.3 Cellular Component: cytoplasm; intracellular membrane-bound organelle; nuclear speck; nucleoplasm; nucleus; protein complex Molecular Function:AT DNA binding; miRNA binding; protein binding; protein heterodimerization activity; transcription activator binding; transcription factor activity Biological Process: B cell homeostasis; B cell proliferation; B cell receptor signaling pathway; blood vessel development; blood vessel remodeling; chondrocyte differentiation; endochondral ossification; germinal center formation; heart development; heart looping; humoral immune response; learning and/or memory; MAPKKK cascade; melanocyte differentiation; muscle cell fate determination; muscle development; myotube differentiation; negative regulation of neuron apoptosis; negative regulation of ossification; negative regulation of transcription from RNA polymerase II promoter; nervous system development; neural crest cell differentiation; neuron development; neuron differentiation; neuron migration; neuron morphogenesis during differentiation; osteoblast differentiation; platelet formation; positive regulation of B cell proliferation; positive regulation of bone mineralization; positive regulation of cardiac muscle cell proliferation; positive regulation of muscle cell differentiation; positive regulation of myoblast differentiation; positive regulation of neuron differentiation; positive regulation of osteoblast differentiation; positive regulation of skeletal muscle development; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of excitatory postsynaptic membrane potential; regulation of germinal center formation; regulation of megakaryocyte differentiation; regulation of neuron apoptosis; regulation of neurotransmitter secretion; regulation of synaptic activity; regulation of synaptic plasticity; regulation of synaptic transmission, glutamatergic; regulation of synaptogenesis; regulation of transcription, DNA-dependent; response to virus; skeletal muscle development; smooth muscle cell differentiation; ventricular cardiac muscle cell differentiation Disease: Mental Retardation, Autosomal Dominant 20
NCBI Summary:	This locus encodes a member of the MADS box transcription enhancer factor 2 (MEF2) family of proteins, which play a role in myogenesis. The encoded protein, MEF2 polypeptide C, has both trans-activating and DNA binding activities. This protein may play a role in maintaining the differentiated state of muscle cells. Mutations and deletions at this locus have been associated with severe mental retardation, stereotypic movements, epilepsy, and cerebral malformation. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2010]
UniProt Code:	Q06413
NCBI GenInfo Identifier:	2500875
NCBI Gene ID:	4208
NCBI Accession:	Q06413.1
UniProt Secondary Accession:	Q06413,C9JMZ0, D7F7N5, F8W7V7,
UniProt Related Accession:	Q06413
Molecular Weight:	50,242 Da
NCBI Full Name:	Myocyte-specific enhancer factor 2C
NCBI Synonym Full Names:	myocyte enhancer factor 2C
NCBI Official Symbol:	MEF2C
NCBI Official Synonym Symbols:	DEL5q14.3; C5DELq14.3
NCBI Protein Information:	myocyte-specific enhancer factor 2C
UniProt Protein Name:	Myocyte-specific enhancer factor 2C
Protein Family:	Myocyte-specific enhancer factor
UniProt Gene Name:	MEF2C
UniProt Entry Name:	MEF2C_HUMAN

	Western blot analysis of extracts of mouse brain, using MEF2C antibody (CAB12385) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human placenta using MEF2C antibody (CAB12385) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human endometrium cancer using MEF2C antibody (CAB12385) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of mouse brain using MEF2C Polyclonal Antibody (CAB12385) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-MEF2C Antibody (CAB2585)	MEF2C (Phospho-Ser396) Transcription Factor Activity Assay
Anti-Phospho-MEF2C-S396 Antibody (CABP0075)	MEF2C (Phospho-Ser396) Colorimetric Cell-Based ELISA Kit
MEF2C Antibody (PACO02898)
Phospho-MEF2C (S396) Antibody (PACO04316)
Phospho-MEF2C (S387) Antibody (PACO04317)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)