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Phospho-p90Rsk/RSK1/RPS6KA1-S363 Rabbit Polyclonal Antibody (CABP0767)

SKU:
CABP0767
Product Type:
Antibody
Applications:
WB
IP
Reactivity:
Human
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
€419
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Description

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Product Name:Phospho-p90Rsk/RSK1/RPS6KA1-S363 Rabbit Polyclonal Antibody
SKU:CABP0767
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Rat
Immunogen:A synthetic phosphorylated peptide around S363 of human p90Rsk/RSK1/RPS6KA1 (NP_002944.2).
Sequence:KDSP G
Tested Applications:WB IP ELISA
Recommended Dilution:WB,1:500 - 1:1000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:RSK; HU-1; RSK1; p90Rsk; MAPKAPK1; MAPKAPK1A; Phospho-p90Rsk/RSK1/RPS6KA1-S363
Positive Sample:HeLa
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:83kDa
Observed MW:90kDa

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Purification Method:Affinity purification
Gene ID:6195
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using (CABP0767) at 1:1000 dilution.HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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