Western blot analysis of 293T cells, using Phospho-PKC alpha-T638 antibody (CABP1376) at 1:7000 dilution. 293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The Phospho-PKC alpha-T638 Monoclonal Antibody (CABP1376) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody specifically targets the phosphorylated form of PKC alpha at threonine 638, a critical site for its activation.Raised in mice, this monoclonal antibody is highly sensitive and specific for detecting phosphorylated PKC alpha in human samples. It is validated for use in Western blot and immunohistochemistry applications, enabling researchers to analyze the activation status of PKC alpha in various tissues and cell types.PKC alpha is implicated in various diseases, including cancer, cardiovascular disorders, and neurological conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Phospho-PKC alpha-T638 Monoclonal Antibody
SKU:
CABP1376
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC58020
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:2000 - 1:8000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.
Purification Method
Affinity purification
Gene ID
5578
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from 293T cells, using Phospho-PKC alpha-T638 Rabbit mAb (CABP1376) at 1:7000 dilution. 293T cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 180s.
