The Phospho-VASP (S157) Polyclonal Antibody (CABP0763) is a valuable tool for researchers studying vasodilator-stimulated phosphoprotein (VASP) phosphorylation at serine 157. VASP is a key regulator of actin dynamics and cell migration, and phosphorylation at S157 is known to be involved in cell motility and cytoskeletal organization.Raised in rabbits, this antibody is highly specific for detecting phosphorylated VASP at S157 in human samples, making it ideal for Western blotting applications. By targeting this specific phosphorylation site, researchers can gain insights into the signaling pathways and molecular mechanisms controlling VASP activity in various cellular processes.
With its versatility and reliability in detecting phospho-VASP S157, this antibody is a valuable tool for studies in cell biology, cancer research, and other fields where understanding VASP phosphorylation dynamics is essential. Investigating the role of VASP S157 phosphorylation can provide crucial insights into the regulation of cell motility, invasion, and other cellular processes, offering new perspectives for therapeutic interventions in diseases like cancer and cardiovascular disorders.
Product Name:
Phospho-VASP-S157 Rabbit Polyclonal Antibody
SKU:
CABP0763
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S157 of human Phospho-VASP-S157 (NP_003361.1).
Sequence:
RVSN A
Tested Applications:
WBIHC-PIPELISA
Recommended Dilution:
WB,1:500 - 1:2000 IHC-P,1:50 - 1:100 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.
Purification Method:
Affinity purification
Gene ID:
7408
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HEL cells using Phospho-VASP-S157 Rabbit pAb (CABP0763) at 1:1000 dilution. HEL cells were treated by Forskolin (20 uM) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 0.8s.
