Anti-PIK3C2A Antibody (CAB8526)

Antibody Name:	Anti-PIK3C2A Antibody
Antibody SKU:	CAB8526
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PIK3C2A (NP_002636.2).

Application:	WB IHC IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	SKOV3, HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human PIK3C2A (NP_002636.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAQI SSNS GFKE CPSS HPEP TRAK DVDK EEAL QMEA EALA KLQK DRQV TDNQ RGFE LSSS TRKK AQVY NKQD YDLM VFPE SDSQ KRAL DIDV EKLT QAEL EKLL LDDS FETK KTPV LPVT PILS PSFS AQLY FRPT IQRG QWPP GLPG PSTY ALPS IYPS TYSK QAAF QNGF NPRM PTFP STEP IYLS LPGQ SPYF SYPL
Gene ID:	5286
Uniprot:	O00443
Cellular Location:	Cell membrane, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Nucleus, clathrin-coated vesicle
Calculated MW:	53kDa/190kDa
Observed MW:	191kDa

Synonyms:

PIK3C2A, CPK, PI3-K-C2(ALPHA), PI3-K-C2A, PI3K-C2-alpha, PI3K-C2alpha

Background:

The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling.

UniProt Protein Function:	PIK3C2A: a protein of the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. Its PI3-kinase is not sensitive to nanomolar levels of the inhibitor wortmannin. Activated by insulin and may be involved in integrin-dependent signaling.
UniProt Protein Details:	Protein type:Carbohydrate Metabolism - inositol phosphate; Autophagy; Kinase, lipid; EC 2.7.1.154; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 11p15.5-p14 Cellular Component: cytoplasm; cytosol; membrane; phosphoinositide 3-kinase complex; plasma membrane; vesicle Molecular Function:1-phosphatidylinositol-3-kinase activity; phosphatidylinositol-4-phosphate 3-kinase activity; phosphoinositide 3-kinase activity Biological Process: clathrin cage assembly; endocytosis; epidermal growth factor receptor signaling pathway; exocytosis; insulin receptor signaling pathway; phosphatidylinositol biosynthetic process; platelet-derived growth factor receptor signaling pathway; vascular smooth muscle contraction
NCBI Summary:	The protein encoded by this gene belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The PI3-kinase activity of this protein is not sensitive to nanomolar levels of the inhibitor wortmanin. This protein was shown to be able to be activated by insulin and may be involved in integrin-dependent signaling. [provided by RefSeq, Jul 2008]
UniProt Code:	O00443
NCBI GenInfo Identifier:	152031652
NCBI Gene ID:	5286
NCBI Accession:	O00443.2
UniProt Secondary Accession:	O00443,Q14CQ9, B0LPH2, B4E2G4,
UniProt Related Accession:	O00443
Molecular Weight:	53,742 Da
NCBI Full Name:	Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha
NCBI Synonym Full Names:	phosphatidylinositol-4-phosphate 3-kinase catalytic subunit type 2 alpha
NCBI Official Symbol:	PIK3C2A
NCBI Official Synonym Symbols:	CPK; PI3-K-C2A; PI3K-C2alpha; PI3K-C2-alpha; PI3-K-C2(ALPHA)
NCBI Protein Information:	phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha
UniProt Protein Name:	Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha
UniProt Synonym Protein Names:	Phosphoinositide 3-kinase-C2-alpha
Protein Family:	Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing
UniProt Gene Name:	PIK3C2A
UniProt Entry Name:	P3C2A_HUMAN

	Western blot analysis of extracts of various cell lines, using PIK3C2A antibody (CAB8526) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse heart using PIK3C2A antibody (CAB8526) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat heart using PIK3C2A antibody (CAB8526) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver damage using PIK3C2A antibody (CAB8526) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using PIK3C2A antibody (CAB8526) at dilution of 1:100. Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)