Anti-PIK3R3 Antibody (CAB17322)

Antibody Name:	Anti-PIK3R3 Antibody
Antibody SKU:	CAB17322
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant protein of human PIK3R3

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	U-251MG, PC-3, 293T

Immunogen:	Recombinant protein of human PIK3R3
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	8503
Uniprot:	Q92569
Cellular Location:
Calculated MW:	43kDa/47kDa/54kDa
Observed MW:	60kDa

Synonyms:

PIK3R3, p55, p55-GAMMA, p55PIK

Background:

Phosphatidylinositol 3-kinase (PI3K) phosphorylates phosphatidylinositol and similar compounds, which then serve as second messengers in growth signaling pathways. PI3K is composed of a catalytic and a regulatory subunit. The protein encoded by this gene represents a regulatory subunit of PI3K. The encoded protein contains two SH2 domains through which it binds activated protein tyrosine kinases to regulate their activity.

UniProt Protein Function:	PIK3R3: Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1. Heterodimer of a regulatory subunit PIK3R3 and a p110 catalytic subunit (PIK3CA, PIK3CB or PIK3CD). Interacts with AXL. Highest levels in brain and testis. Lower levels in adipose tissue, kidney, heart, lung and skeletal muscle. Belongs to the PI3K p85 subunit family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; Kinase, lipid Chromosomal Location of Human Ortholog: 1p34.1 Cellular Component: cytosol; phosphoinositide 3-kinase complex Molecular Function:protein binding; 1-phosphatidylinositol-3-kinase activity; phosphoinositide 3-kinase regulator activity Biological Process: phospholipid metabolic process; phosphatidylinositol biosynthetic process; insulin receptor signaling pathway; regulation of phosphoinositide 3-kinase activity
NCBI Summary:	Phosphatidylinositol 3-kinase (PI3K) phosphorylates phosphatidylinositol and similar compounds, which then serve as second messengers in growth signaling pathways. PI3K is composed of a catalytic and a regulatory subunit. The protein encoded by this gene represents a regulatory subunit of PI3K. The encoded protein contains two SH2 domains through which it binds activated protein tyrosine kinases to regulate their activity. [provided by RefSeq, Jun 2016]
UniProt Code:	Q92569
NCBI GenInfo Identifier:	317373310
NCBI Gene ID:	8503
NCBI Accession:	Q92569.2
UniProt Related Accession:	Q92569
Molecular Weight:
NCBI Full Name:	Phosphatidylinositol 3-kinase regulatory subunit gamma
NCBI Synonym Full Names:	phosphoinositide-3-kinase regulatory subunit 3
NCBI Official Symbol:	PIK3R3
NCBI Official Synonym Symbols:	p55; p55PIK; p55-GAMMA
NCBI Protein Information:	phosphatidylinositol 3-kinase regulatory subunit gamma
UniProt Protein Name:	Phosphatidylinositol 3-kinase regulatory subunit gamma
UniProt Synonym Protein Names:	Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; PI3-kinase subunit p55-gamma; PtdIns-3-kinase regulatory subunit p55-gamma; p55PIK
UniProt Gene Name:	PIK3R3
UniProt Entry Name:	P55G_HUMAN

	Western blot analysis of extracts of various cell lines, using PIK3R3 antibody (CAB17322) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of NIH-3T3 cells using PIK3R3 antibody (CAB17322) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH-3T3 cells using PIK3R3 antibody (CAB17322) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-PIK3R3 Antibody (CAB7942)

Anti-PIK3R3 Antibody (CAB17112)

Anti-PI 3 Kinase p55 gamma Antibody (CAB3980)

PIK3R3 Antibody (PACO11249)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)