Anti-PKM Antibody (MACO0619)

Product Name:	Anti-PKM Antibody
Product Sku:	MACO0619
Size:	50ul
Host Species:	Mouse
Tested Applications:	ELISA, WB, IHC, IF, FC, IP
Recommended Dilutions:	WB:1:4000-1:256000, IHC:1:200-1:500, IF:1:150-1:300, FC:1:50-1:200, IP:1Âµl-4Âµl
Species Reactivity:	Human, Rat, Mouse, Rabbit
Immunogen:	Recombinant Human Pyruvate kinase PKM protein (2-531AA)

Form:	Liquid
Storage Buffer:	Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:	>95%, Protein G purified
Clonality:	Monoclonal
Isotype:	IgG1
Conjugate:	Non-conjugated
Background:	Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
Synonyms:	CTHBP antibody; Cytosolic thyroid hormone-binding protein antibody; KPYM_HUMAN antibody; OIP-3 antibody; Opa-interacting protein 3 antibody; p58 antibody; pkm antibody; PKM1 antibody; PKM2 antibody; Pyruvate kinase 2/3 antibody; Pyruvate kinase muscle isozyme antibody; Pyruvate kinase PKM antibody; THBP1 antibody; Thyroid hormone-binding protein 1 antibody; Tumor M2-PK antibody

	Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate All lanes: PKM antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 58 kDa Observed band size: 58 KDa Exposure time: 1min
	Western Blot Positive WB detected in: Mouse Heart tissue, Mouse Brain tissue, Mouse Skeletal Muscle tissue All lanes: PKM antibody at 1:4000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 55-60 kDa Observed band size: 55-60 kDa
	Western Blot Positive WB detected in: A549 whole cell lysate, 293T whole cell lysate, U251 whole cell lysate, Rat Brain tissue, Rabbit Skeletal Muscle tissue All lanes: PKM antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 58 kDa Observed band size: 58 KDa Exposure time: 1min

UniProt Protein Function:	PKM2: one of three rate-limiting enzymes in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule ATP and a pyruvate molecule, which is a central metabolic intermediate that can be used as a building block or oxidized further. Overexpressed in a variety of cancers. Interacts with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. Binds Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogens is. There are several mammalian isozymes of pyruvate kinase encoded by different genes. The L type predominates in liver, and the M type in muscle and brain. Two alternatively spliced human isoforms of PKM2 have been reported.
UniProt Protein Details:	Protein type:Carbohydrate Metabolism - glycolysis and gluconeogenesis; Kinase, other; EC 2.7.1.40; Carbohydrate Metabolism - pyruvate; Mitochondrial; Nucleotide Metabolism - purine Chromosomal Location of Human Ortholog: 15q22 Cellular Component: extracellular matrix; mitochondrion; cytoplasm; plasma membrane; nucleus; cytosol; vesicle; cilium Molecular Function:potassium ion binding; protein binding; magnesium ion binding; pyruvate kinase activity; ATP binding Biological Process: glycolysis; carbohydrate metabolic process; programmed cell death; glucose metabolic process; pathogenesis; phosphorylation
NCBI Summary:	This gene encodes a protein involved in glycolysis. The encoded protein is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Several alternatively spliced transcript variants encoding a few distinct isoforms have been reported. [provided by RefSeq, May 2011]
UniProt Code:	P14618
NCBI GenInfo Identifier:	20178296
NCBI Gene ID:	5315
NCBI Accession:	P14618.4
UniProt Secondary Accession:	P14618,P14786, Q53GK4, Q96E76, Q9BWB5, Q9UCV6, Q9UPF2 A6NFK3, B2R5N8, B3KRY0, B4DFX8, B4DUU6,
UniProt Related Accession:	P14618
Molecular Weight:
NCBI Full Name:	Pyruvate kinase PKM
NCBI Synonym Full Names:	pyruvate kinase, muscle
NCBI Official Symbol:	PKM
NCBI Official Synonym Symbols:	PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
NCBI Protein Information:	pyruvate kinase PKM; p58; OIP-3; tumor M2-PK; PK, muscle type; pyruvate kinase 2/3; OPA-interacting protein 3; pyruvate kinase isozymes M1/M2; pyruvate kinase muscle isozyme; thyroid hormone-binding protein 1; epididymis secretory protein Li 30; cytosolic thyroid hormone-binding protein; thyroid hormone-binding protein, cytosolic
UniProt Protein Name:	Pyruvate kinase PKM
UniProt Synonym Protein Names:	Cytosolic thyroid hormone-binding protein; CTHBP; Opa-interacting protein 3; OIP-3; Pyruvate kinase 2/3; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; THBP1; Tumor M2-PK; p58
Protein Family:	Pyruvate kinase
UniProt Gene Name:	PKM
UniProt Entry Name:	KPYM_HUMAN

Antibodies	ELISA Kits
Anti-PKM Antibody (CAB0268)	Human PKM2 ELISA Kit
Anti-PKM Mouse Monoclonal Antibody (CAB0540)	Human PKM2 (Pyruvate Kinase, Muscle) CLIA Kit (HUES00639)
Anti-PKM Antibody (CAB13905)	Human Pyruvate kinase isozymes M1/M2 (PKM2) ELISA Kit
Anti-PKM Antibody (CAB16700)
Anti-Phospho-Pyruvate kinase PKM2-S37 Antibody (CABP0428)

Secondary Antibody

Anti-HRP Goat Anti-Mouse IgG (H+L) Antibody (CABS003)