Anti-POLD1 Antibody (CAB5323)

Antibody Name:	Anti-POLD1 Antibody
Antibody SKU:	CAB5323
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 808-1107 of human POLD1 (NP_002682.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	HeLa, SW480, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 808-1107 of human POLD1 (NP_002682.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RYAG LLFS SRPD AHDR MDCK GLEA VRRD NCPL VANL VTAS LRRL LIDR DPEG AVAH AQDV ISDL LCNR IDIS QLVI TKEL TRAA SDYA GKQA HVEL AERM RKRD PGSA PSLG DRVP YVII SAAK GVAA YMKS EDPL FVLE HSLP IDTQ YYLE QQLA KPLL RIFE PILG EGRA EAVL LRGD HTRC KTVL TGKV GGLL AFAK RRNC CIGC RTVL SHQG AVCE FCQP RESE LYQK EVSH LNAL EERF SRLW TQCQ RCQG SLHE DVIC TSRD CPIF YMRK KVRK DLED QEQL LRRF GPPG PEAW
Gene ID:	5424
Uniprot:	P28340
Cellular Location:	Nucleus
Calculated MW:	123kDa
Observed MW:	130kDa

Synonyms:	POLD1, CDC2, CRCS10, MDPL, POLD
Background:	This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6.

UniProt Protein Function:	POLD1: Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex. Belongs to the DNA polymerase type-B family.
UniProt Protein Details:	Protein type:Transferase; EC 2.7.7.7; Nucleotide Metabolism - pyrimidine; Hydrolase; DNA replication; DNA repair, damage; Nucleotide Metabolism - purine Chromosomal Location of Human Ortholog: 19q13.3 Cellular Component: cytoplasm; delta DNA polymerase complex; membrane; nuclear chromosome, telomeric region; nucleoplasm; nucleotide-excision repair complex; nucleus Molecular Function:3'-5'-exodeoxyribonuclease activity; chromatin binding; damaged DNA binding; DNA binding; DNA-directed DNA polymerase activity; protein binding Biological Process: base-excision repair, gap-filling; bypass DNA synthesis; DNA damage response, detection of DNA damage; DNA repair; DNA replication; DNA replication proofreading; DNA strand elongation during DNA replication; DNA synthesis during DNA repair; fatty acid homeostasis; mismatch repair; nucleotide-excision repair, DNA gap filling; nucleotide-excision repair, DNA incision; nucleotide-excision repair, DNA incision, 5'-to lesion; response to UV; telomere maintenance; telomere maintenance via recombination; transcription-coupled nucleotide-excision repair Disease: Colorectal Cancer, Susceptibility To, 10; Mandibular Hypoplasia, Deafness, Progeroid Features, And Lipodystrophy Syndrome
NCBI Summary:	This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6. [provided by RefSeq, Mar 2012]
UniProt Code:	P28340
NCBI GenInfo Identifier:	50403732
NCBI Gene ID:	5424
NCBI Accession:	P28340.2
UniProt Secondary Accession:	P28340,Q8NER3, Q96H98,
UniProt Related Accession:	P28340
Molecular Weight:	123,631 Da
NCBI Full Name:	DNA polymerase delta catalytic subunit
NCBI Synonym Full Names:	DAN polymerase delta 1, catalytic subunit
NCBI Official Symbol:	POLD1
NCBI Official Synonym Symbols:	CDC2; MDPL; POLD; CRCS10
NCBI Protein Information:	DNA polymerase delta catalytic subunit
UniProt Protein Name:	DNA polymerase delta catalytic subunit
UniProt Synonym Protein Names:	DNA polymerase subunit delta p125
Protein Family:	DNA polymerase
UniProt Gene Name:	POLD1
UniProt Entry Name:	DPOD1_HUMAN

	Western blot analysis of extracts of various cell lines, using POLD1 antibody (CAB5323) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using POLD1 antibody (CAB5323). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of A549 cells using POLD1 antibody (CAB5323).
	Immunofluorescence analysis of U2OS cells using POLD1 antibody (CAB5323). (Green)gamma H2AX (phospho S139) used for DNA damage marker. Blue: DAPI for nuclear staining. Cells were treated by UV-A laser.

Antibodies

Anti-POLD1 Antibody (CAB4218)

POLD1 Antibody (PACO04631)

POLD1 Antibody (PACO11342)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)