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Anti-NDRG1 Antibody (CAB2142)

SKU:
CAB2142
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
  • Cell Biology Antibodies 8 Anti-NDRG1 Antibody CAB2142
  • Cell Biology Antibodies 8 Anti-NDRG1 Antibody CAB2142
€139 - €419
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Description

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Antibody Name:Anti-NDRG1 Antibody
Antibody SKU:CAB2142
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 175-394 of human NDRG1 (NP_001128714.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, SW480, PC-3, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 175-394 of human NDRG1 (NP_001128714.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:WAAS KISG WTQA LPDM VVSH LFGK EEMQ SNVE VVHT YRQH IVND MNPG NLHL FINA YNSR RDLE IERP MPGT HTVT LQCP ALLV VGDS SPAV DAVV ECNS KLDP TKTT LLKM ADCG GLPQ ISQP AKLA EAFK YFVQ GMGY MPSA SMTR LMRS RTAS GSSV TSLD GTRS RSHT SEGT RSRS HTSE GTRS RSHT SEGA HLDI TPNS GAAG NSAG PKSM EVSC
Gene ID:10397
Uniprot:Q92597
Cellular Location:Cell membrane, Cytoplasm, Nucleus, centrosome, cytoskeleton, cytosol, microtubule organizing center
Calculated MW:33kDa/35kDa/42kDa
Observed MW:48kDa
Synonyms:NDRG1, CAP43, CMT4D, DRG-1, DRG1, GC4, HMSNL, NDR1, NMSL, PROXY1, RIT42, RTP, TARG1, TDD5
Background:This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
UniProt Protein Function:NDRG1: a hypoxia-inducible anti-apoptotic protein. Promotes proliferation and correlates with invasion, metastasis and poor prognosis in many cancers. Is involved in growth arrest and cell differentiation during development. A ubiquitous Rab4a effector protein that modulates angiogenesis and is involved in vesicular recycling of E-cadherin. Colocalizes with transferrin during the recycling in cells. NDRG1 knockdown delays the recycling rate of transferrin, while its overexpression increases the rate of transferrin recycling. Interacts with SIRT1/p53 signaling to attenuate hypoxic injury in human trophoblasts. Its expression is induced by an elevation of free intracellular calcium ion caused by nickel ion exposure. Highly expressed in placental membranes, prostate, kidney, small intestine, ovary, peripheral nerves and Schwann cells. Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D).
UniProt Protein Details:

Protein type:Cell development/differentiation; Vesicle

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: microtubule cytoskeleton; microtubule; centrosome; cell-cell adherens junction; recycling endosome membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; cytosol; nucleus

Molecular Function:protein binding; gamma-tubulin binding; cadherin binding; microtubule binding; Rab GTPase binding

Biological Process: response to metal ion; DNA damage response, signal transduction by p53 class mediator; myelin maintenance in the peripheral nervous system; mast cell activation

Disease: Charcot-marie-tooth Disease, Type 4d

NCBI Summary:This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
UniProt Code:Q92597
NCBI GenInfo Identifier:6166568
NCBI Gene ID:10397
NCBI Accession:Q92597.1
UniProt Secondary Accession:Q92597,O15207, Q6IBG2, Q9NYR6, Q9UK29, B3KR80, B7Z446
UniProt Related Accession:Q92597
Molecular Weight:394
NCBI Full Name:Protein NDRG1
NCBI Synonym Full Names:N-myc downstream regulated 1
NCBI Official Symbol:NDRG1  
NCBI Official Synonym Symbols:GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1  
NCBI Protein Information:protein NDRG1; protein regulated by oxygen-1; differentiation-related gene 1 protein; nickel-specific induction protein Cap43; N-myc downstream-regulated gene 1 protein; reducing agents and tunicamycin-responsive protein
UniProt Protein Name:Protein NDRG1
UniProt Synonym Protein Names:Differentiation-related gene 1 protein; DRG-1; N-myc downstream-regulated gene 1 protein; Nickel-specific induction protein Cap43; Reducing agents and tunicamycin-responsive protein; RTP; Rit42
Protein Family:Protein
UniProt Gene Name:NDRG1  
UniProt Entry Name:NDRG1_HUMAN
Anti-NDRG1 Antibody (CAB2142)Western blot analysis of extracts of various cell lines, using NDRG1 antibody (CAB2142) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).
Anti-NDRG1 Antibody (CAB2142)Immunofluorescence analysis of U2OS cells using NDRG1 antibody (CAB2142). Blue: DAPI for nuclear staining.

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