Anti-PSMD4 Antibody (CAB1061)

Antibody Name:	Anti-PSMD4 Antibody
Antibody SKU:	CAB1061
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat, Monkey
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-377 of human PSMD4 (NP_002801.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat, Monkey
Positive Samples:	HUVEC

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-377 of human PSMD4 (NP_002801.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MVLE STMV CVDN SEYM RNGD FLPT RLQA QQDA VNIV CHSK TRSN PENN VGLI TLAN DCEV LTTL TPDT GRIL SKLH TVQP KGKI TFCT GIRV AHLA LKHR QGKN HKMR IIAF VGSP VEDN EKDL VKLA KRLK KEKV NVDI INFG EEEV NTEK LTAF VNTL NGKD GTGS HLVT VPPG PSLA DALI SSPI LAGE GGAM LGLG ASDF EFGV DPSA DPEL ALAL RVSM EEQR QRQE EEAR RAAA ASAA EAGI ATTG TEDS DDAL LKMT ISQQ EFGR TGLP DLSS MTEE EQIA YAMQ MSLQ GAEF GQAE SADI DASS AMDT SEPA KEED DYDV MQDP EFLQ SVLE NLPG VDPN NEAI RNAM GSLA SQAT KDGK KDKK EEDK K
Gene ID:	5710
Uniprot:	P55036
Cellular Location:
Calculated MW:	28kDa/40kDa
Observed MW:	50kDa

Synonyms:

PSMD4, AF, AF-1, ASF, MCB1, Rpn10, S5A, pUB-R5

Background:

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21.

UniProt Protein Function:	PSMD4: Binds and presumably selects ubiquitin-conjugates for destruction. Displays selectivity for longer polyubiquitin chains. Modulates intestinal fluid secretion. The 26S proteasome is composed of a core protease, known as the 20S proteasome, capped at one or both ends by the 19S regulatory complex (RC). The RC is composed of at least 18 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively. Directly interacts with NUB1. Interacts with SQSTM1. Interacts with UBQLN4. Belongs to the proteasome subunit S5A family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Protease; Proteasome complex Chromosomal Location of Human Ortholog: 1q21.3 Cellular Component: proteasome complex; nucleoplasm; cytoplasm; cytosol Molecular Function:identical protein binding; protein binding Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle
NCBI Summary:	The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. Pseudogenes have been identified on chromosomes 10 and 21. [provided by RefSeq, Jul 2008]
UniProt Code:	P55036
NCBI GenInfo Identifier:	1709796
NCBI Gene ID:	5710
NCBI Accession:	P55036.1
UniProt Secondary Accession:	P55036,Q5VWC5, Q9NS92, D3DV16,
UniProt Related Accession:	P55036
Molecular Weight:	377
NCBI Full Name:	26S proteasome non-ATPase regulatory subunit 4
NCBI Synonym Full Names:	proteasome (prosome, macropain) 26S subunit, non-ATPase, 4
NCBI Official Symbol:	PSMD4
NCBI Official Synonym Symbols:	AF; ASF; S5A; AF-1; MCB1; Rpn10; pUB-R5
NCBI Protein Information:	26S proteasome non-ATPase regulatory subunit 4; angiocidin; antisecretory factor 1; S5a/antisecretory factor protein; multiubiquitin chain-binding protein; 26S proteasome regulatory subunit S5A
UniProt Protein Name:	26S proteasome non-ATPase regulatory subunit 4
UniProt Synonym Protein Names:	26S proteasome regulatory subunit RPN10; 26S proteasome regulatory subunit S5A; Antisecretory factor 1; AF; ASF; Multiubiquitin chain-binding protein
Protein Family:	26S proteasome non-ATPase regulatory
UniProt Gene Name:	PSMD4
UniProt Entry Name:	PSMD4_HUMAN

	Western blot analysis of extracts of HUVEC cells, using PSMD4 antibody (CAB1061) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of H9C2 cells using PSMD4 Rabbit pAb (CAB1061) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U2OS cells using PSMD4 Rabbit pAb (CAB1061) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies

Anti-PSMD4 Antibody (CAB3663)

PSMD4 Antibody (PACO11552)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)