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Western blot analysis of extracts of various cell lines, using PTEN Rabbit mAb antibody (CAB21892) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Key Features

PTEN Monoclonal Antibody (CAB21892)

Datasheet MSDS
SKU CAB21892
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Human, Mouse, Rat
Host Species Rabbit
Synonyms BZS, DEC, CWS1, GLM2, MHAM, TEP1, MMAC1, PTEN1, 10q23del, PTENbeta, PTEN
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Description

system_update_altDatasheetsystem_update_altMSDS

PTEN Monoclonal Antibody (CAB21892)

The PTEN Monoclonal Antibody (CAB21892) is a high-quality antibody developed for reliable detection and analysis of target proteins. This monoclonal antibody, produced in mice, is highly specific to PTEN and has been validated for use in applications such as Western blot and immunohistochemistry.PTEN is a crucial player in the regulation of cell proliferation and survival, and its dysregulation has been linked to various cancers and other diseases.

This antibody is validated for use in WB, IHC-P, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:PTEN Monoclonal Antibody
SKU:CAB21892
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Clone Number:ARC0364
Conjugate:Unconjugated
Immunogen:Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:CSIE RADN DKEY LVLT LTKN DLDK ANKD KANR YFSP NFKV KLYF TKTV EEPS NPEA SSST SVTP DVSD NEPD HYRY SDTT DSDP ENEP FDED QHTQ ITKV
Tested Applications:WB IHC-P ELISA
Recommended Dilution:
WB1:1000 - 1:2000
IHC-P1:200 - 1:800
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:BZS, DEC, CWS1, GLM2, MHAM, TEP1, MMAC1, PTEN1, 10q23del, PTENbeta, PTEN
Positive Sample:MCF7, Mouse kidney, C6
Cellular Localization:Cytoplasm, Nucleus, Pml Body, Secreted.
Calculated MW:47kDa
Observed MW:54kDa

This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.

Purification MethodAffinity purification
Gene ID5728
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates using PTEN Rabbit mAb (CAB21892) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 45s.
Product ImageImmunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using PTEN Rabbit mAb (CAB21892) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
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