Western blot analysis of extracts of various cell lines, using PTPRR antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
The PTPRR Monoclonal Antibody (CAB20981) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, generated in rabbits, has high specificity and sensitivity for human samples, making it suitable for a range of applications including Western blotting. By targeting the PTPRR protein, researchers can detect and analyze its expression in different cell types, facilitating investigations into the role of PTPRR in biological pathways such as cell signaling and development.PTPRR, also known as protein tyrosine phosphatase receptor type R, is involved in regulating phosphorylation events and modulating cellular responses to external stimuli.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
PTPRR Monoclonal Antibody
SKU:
CAB20981
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Clone Number:
ARC2973
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PTPRQ, EC-PTP, PCPTP1, PTP-SL, PTPBR7, PTPRR
Positive Sample:
Mouse brain, Rat brain
Cellular Localization:
Cell Membrane, Cytoplasm, Single-Pass Type I Membrane Protein, Perinuclear Region, Perinuclear Region.
Calculated MW:
74kDa
Observed MW:
74kDa
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single intracellular catalytic domain, and thus represents a receptor-type PTP. Silencing of this gene has been associated with colorectal cancer. Multiple transcript variants encoding different isoforms have been found for this gene. This gene shares a symbol (PTPRQ) with another gene, protein tyrosine phosphatase, receptor type, Q (GeneID 374462), which is also located on chromosome 12.
Purification Method
Affinity purification
Gene ID
5801
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using PTPRR Rabbit mAb (CAB20981) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
