The QuickStep Bovine Cortisol ELISA Kit is a premium assay designed for the precise measurement of cortisol levels in bovine serum, plasma, and saliva samples. This kit boasts exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research applications.Cortisol is a key hormone involved in stress response, metabolism, and immune function in bovine species. Monitoring cortisol levels is crucial for studying stress-related conditions, reproductive health, and overall animal well-being.
The QuickStep Bovine Cortisol ELISA Kit provides researchers with a valuable tool for investigating these physiological processes and developing targeted interventions for livestock management.Don't compromise on the quality of your data - choose the QuickStep Bovine Cortisol ELISA Kit for reliable and trustworthy results in your bovine research studies.
Product Name:
QuickStep Bovine Cortisol ELISA Kit
Product Code:
QSES002
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Bovine
Sensitivity:
2.54 ng/mL
Range:
6.25-400 ng/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Cortisol
Application:
This ELISA kit applies to the in vitro quantitative determination of Cortisol concentrations in serum and plasma.
Specificity:
This kit recognizes Cortisol in samples. No significant cross-reactivity or interference between Cortisol and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Bovine serum, plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Bovine Cortisol. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Bovine Cortisol are added to the micro ELISA plate wells. Bovine Cortisol in samples (or standards) competes with a fixed amount of Bovine Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Bovine Cortisol. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Bovine Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (ng/mL)
18.43
58.92
190.43
17.61
58.29
196.43
Standard deviation
1.19
2.56
6.00
1.03
2.86
7.27
CV (%)
6.43
4.34
3.15
5.87
4.90
3.70
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
87-101
92
EDTA plasma(n=8)
93-107
98
Samples were spiked with high concentrations of Bovine Cortisol and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
