The Rat ADP (Adiponectin) ELISA Kit is specifically designed for the quantitative measurement of adiponectin levels in rat serum, plasma, and tissue homogenates. This kit offers high sensitivity and precision, guaranteeing accurate and consistent results for a variety of research purposes.Adiponectin is a key adipokine that plays a vital role in regulating glucose and lipid metabolism, as well as inflammation and insulin sensitivity. Dysregulation of adiponectin levels has been linked to various metabolic disorders such as obesity, type 2 diabetes, and cardiovascular diseases, making it a valuable marker for studying these conditions and potential therapeutic interventions.
With easy-to-follow protocols and efficient assay performance, the Rat ADP ELISA Kit is a valuable tool for researchers exploring the role of adiponectin in metabolic diseases and seeking new insights into potential treatment strategies.
Product Name:
QuickStep Rat Adiponectin ELISA Kit
Product Code:
QSES053
Assay Type:
Sandwich
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Rat
Sensitivity:
6.43 ng/mL
Range:
31.25-1000 ng/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Adiponectin
Application:
This ELISA kit applies to the in vitro quantitative determination of ADP/Acrp30 concentrations in serum and plasma.
Specificity:
This kit recognizes ADP/Acrp30 in samples. No significant cross-reactivity or interference between ADP/Acrp30 and analogues was observed.
Dilution Method:
It is recommended to dilute normal serum/plasma samples at 50 fold.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ADP/Acrp30. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rat ADP/Acrp30 are added to the micro ELISA plate wells. Rat ADP/Acrp30 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Rat ADP/Acrp30. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Rat ADP/Acrp30. The concentration of Rat ADP/Acrp30 in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (ng/mL)
52.94
153.56
371.41
50.14
155.02
346.08
Standard deviation
2.94
8.06
11.14
2.58
8.79
17.65
CV (%)
5.55
5.25
3.00
5.15
5.67
5.10
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
87-100
95
EDTA plasma(n=8)
95-111
101
Samples were spiked with high concentrations of Rat ADP/Acrp30 and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
