The QuickStep Sheep Testosterone ELISA Kit is a cutting-edge product designed for the precise measurement of testosterone levels in sheep serum, plasma, and other biological samples. This kit offers outstanding sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.Testosterone is a vital hormone in sheep physiology, playing a significant role in reproductive processes, growth, and overall health. Monitoring testosterone levels in sheep is crucial for understanding their reproductive cycles, assessing reproductive performance, and optimizing breeding strategies.
With the QuickStep Sheep Testosterone ELISA Kit, researchers can easily quantify testosterone levels in sheep samples with high precision, making it an indispensable tool for studying sheep reproductive biology, endocrinology, and animal health. Trust in this reliable and efficient ELISA kit for your sheep testosterone analysis needs.
Product Name:
QuickStep Sheep Testosterone ELISA Kit
Product Code:
QSES060
Assay Type:
Competitive
Assay Format:
96T
Assay Time:
1.5h
Reactivity:
Sheep
Sensitivity:
0.25 ng/mL
Range:
0.63-40 ng/mL
Detection Method:
Colormetric
Sample Type:
Serum and plasma
Target:
Testosterone
Application:
This ELISA kit applies to the in vitro quantitative determination of T concentrations in serum and plasma.
Specificity:
This kit recognizes T in samples. No significant cross-reactivity or interference between T and analogues was observed.
Dilution Method:
It is recommended to do the experiment with undiluted Sheep serum and plasma samples.
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Storage & Stability:
An unopened kit can be stored at 2-8°C for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Sheep T. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Sheep T are added to the micro ELISA plate wells. Sheep T in samples (or standards) competes with a fixed amount of Sheep T on the solid phase supporter for sites on the HRP linked detection antibody specific to Sheep T. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Sheep T in the samples is then determined by comparing the OD of the samples to the standard curve.
Item
Specifications
Storage
Micro ELISA Plate(Dismountable)
8 wells - 12 strips
2-8°C, 1 months
Reference Standard
2 vials
2-8°C, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100-)
1 vial, 60 µL
2-8°C(Protect from light)
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C
HRP Linked Ab Diluent
1 vial, 14 mL
Concentrated Wash Buffer(25-)
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
2-8°C(Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Product Description
1 copy
Certificate of Analysis
1 copy
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean (ng/mL)
2.20
5.35
14.06
1.99
5.70
14.18
Standard deviation
0.15
0.24
0.55
0.13
0.24
0.46
CV (%)
6.71
4.52
3.93
6.59
4.26
3.22
Sample Type
Range (%)
Average Recovery (%)
Serum(n=8)
93-107
99
EDTA plasma(n=8)
84-97
91
Samples were spiked with high concentrations of Sheep T and diluted with Reference Standard & Sample Diluent to produceSamples with values within the range of the assay.
