The Rabbit Control IgG (CABC005) is a high-quality antibody developed for reliable detection and analysis of target proteins. The protein encoded by this gene is a transcriptional regulator and tumor suppressor, serving as an activator of genes involved in both innate and acquired immune responses. The encoded protein activates the transcription of genes involved in the body's response to viruses and bacteria, playing a role in cell proliferation, apoptosis, the immune response, and DNA damage response. This protein represses the transcription of several other genes. As a tumor suppressor, it both suppresses tumor cell growth and stimulates an immune response against tumor cells. Defects in this gene have been associated with gastric cancer, myelogenous leukemia, and lung cancer.
This antibody is validated for use in IP, ChIP applications and has demonstrated reactivity against - samples.
Product Name:
Rabbit Control IgG
SKU:
CABC005
Size:
100μL, 200μL, 50μL
Conjugate:
Unconjugated
Immunogen:
This information is considered to be commercially sensitive.
Tested Applications:
IPChIP
Recommended Dilution:
IP
0.5ug-4ug antibody for 200ug-400ug extracts of whole cells
ChIP
5μg antibody for 10μg-15μg of Chromatin
The protein encoded by this gene is a transcriptional regulator and tumor suppressor, serving as an activator of genes involved in both innate and acquired immune responses. The encoded protein activates the transcription of genes involved in the body's response to viruses and bacteria, playing a role in cell proliferation, apoptosis, the immune response, and DNA damage response. This protein represses the transcription of several other genes. As a tumor suppressor, it both suppresses tumor cell growth and stimulates an immune response against tumor cells. Defects in this gene have been associated with gastric cancer, myelogenous leukemia, and lung cancer.
Purification Method
Potein A/G purification
RRID
AB_2771930
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Immunoprecipitation of over-expressed DDDDK-tagged protein in 293T cells incubated using DDDDK-tag antibody (AE063). Secondary antibody: HRP-conjugated AffiniPure Mouse Anti-Rabbit IgG Light Chain (AS061).A mock served as negative control using rabbit Control IgG（CABC005）and over-expressed 293T cell lysate served as positive control.
Chromatin immunoprecipitation was performed with 15 μg of cross-linked chromatin from HeLa cells, using 5 μg of Rabbit Control IgG (CABC005) and CTCF Rabbit pAb (A1133). The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.
