The Rabbit Osteocalcin (OC) ELISA Kit is a highly sensitive and specific tool for detecting levels of osteocalcin in rabbit serum, plasma, and cell culture supernatants. Osteocalcin is a key protein involved in bone formation and turnover, making it an essential marker for studying bone health and diseases such as osteoporosis and osteogenesis imperfecta.This ELISA kit offers accurate and reproducible results, allowing researchers to confidently measure osteocalcin levels in samples.
By understanding the role of osteocalcin in bone metabolism, researchers can gain valuable insight into skeletal health and potentially develop new therapies for bone-related disorders.With its user-friendly design and reliable performance, the Rabbit Osteocalcin (OC) ELISA Kit is an invaluable tool for studying bone biology and advancing research in the field of orthopedics and bone health.
Product Name:
Rabbit Osteocalcin (OC) ELISA Kit
Product Code:
RBDL00056
Size:
96 Assays
Target:
OC
Synonyms:
BGLAP, OT, BGP, Bone Gla Protein, Bone Gamma-Carboxyglutamate Protein, Gamma-carboxyglutamic acid-containing protein
Detection Method:
Sandwich
Range:
0.312-20ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
