The RAD17 Antibody (CAB5359) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to RAD17, this antibody allows for accurate detection and analysis of the protein in various cell types, making it an essential component in studies focused on DNA repair mechanisms and cell cycle regulation.RAD17, a checkpoint protein involved in sensing DNA damage and facilitating repair processes, is essential for maintaining genomic stability and preventing the accumulation of mutations that can lead to cancer development.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
RAD17 Antibody
SKU:
CAB5359
Size:
20μL, 100μL
Reactivity:
Human
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
CCYC, R24L, RAD24, HRAD17, RAD17SP, RAD17
Positive Sample:
SW480, BT-474, K-562, 22Rv1, Raji
Cellular Localization:
Nucleus.
Calculated MW:
77kDa
Observed MW:
77kDa
The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.
Purification Method
Affinity purification
Gene ID
5884
RRID
AB_2766169
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using RAD17 Rabbit pAb (CAB5359) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3s.
Immunoprecipitation analysis of 200 μg extracts of K562 cells using 1 μg RAD17 antibody (CAB5359). Western blot was performed from the immunoprecipitate using RAD17 antibody (CAB5359) at a dilution of 1:1000.
