Rat Acid Phosphatase 5, TartRat e Resistant (ACP5) ELISA Kit
The Rat Acid Phosphatase 5 (Tartrate-Resistant ACP5) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of ACP5 levels in rat serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it ideal for a wide range of research applications.ACP5, also known as tartrate-resistant acid phosphatase 5, is an important enzyme involved in bone remodeling and osteoclast differentiation. It plays a key role in bone resorption and has been implicated in various bone-related disorders such as osteoporosis and Paget's disease.
The measurement of ACP5 levels can help researchers understand the underlying mechanisms of these conditions and develop potential therapeutic interventions.Overall, the Rat Acid Phosphatase 5 (Tartrate-Resistant ACP5) ELISA Kit is a valuable tool for studying bone metabolism and related diseases in rat models, providing researchers with accurate and reliable data for their investigations.
Product Name:
Rat Acid Phosphatase 5, TartRat e Resistant (ACP5) ELISA Kit
Product Code:
RTDL00011
Size:
96 Assays
Target:
ACP5
Synonyms:
TRAP, TrATPase, Tartrate Resistant Acid Phosphatase, Type 5 acid phosphatase
Detection Method:
Sandwich
Range:
0.781-50ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
