The Rat Elastase-2A (ELA2A) ELISA Kit is a powerful tool for accurately measuring levels of elastase-2A in rat biological samples such as serum, plasma, and tissue culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results that are suitable for a wide range of research applications.Elastase-2A is a key enzyme involved in the regulation of inflammatory responses and tissue remodeling. It plays a critical role in various physiological processes and is implicated in conditions such as chronic inflammatory diseases, lung disorders, and cancer.
By accurately measuring elastase-2A levels, researchers can gain valuable insights into disease mechanisms and potential therapeutic targets.In summary, the Rat Elastase-2A (ELA2A) ELISA Kit is a valuable tool for studying the role of elastase-2A in health and disease. Its high sensitivity and specificity make it an essential component of any research aimed at understanding the biology of elastase-2A and developing novel therapeutic interventions.
Product Name:
Rat Elastase 2A (ELA2A) ELISA Kit
Product Code:
RTDL00323
Size:
96 Assays
Target:
ELA2A
Synonyms:
ELA1, PE-1, CELA2A, Chymotrypsin-Like Elastase Family Member 2A
Detection Method:
Sandwich
Range:
62.5-4000pg/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
90-99
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
