The Rat hs-CRP (high-sensitivity C-Reactive Protein) ELISA Kit is meticulously designed to enable the precise and quantitative measurement of high-sensitivity CRP levels, a key biomarker of systemic inflammation and a crucial indicator of various pathological conditions, in diverse biological samples obtained from rat subjects. High-sensitivity CRP serves as an important inflammatory marker indicative of inflammation, infection, and associated adverse health conditions. Accurate quantitation of hs-CRP levels through this specialized ELISA kit plays a critical role in assessing and monitoring inflammatory processes within the body. High levels of hs-CRP are linked to cardiovascular diseases, autoimmune disorders, infections, and several other health complications. Hence, the quantitative data obtained from this ELISA assay can provide valuable insights into the inflammatory status, disease progression, and overall health condition in rat models. The Rat hs-CRP ELISA Kit by Assay Genie delivers exceptional sensitivity and specificity, ensuring accurate, reproducible, and reliable results. Manufactured under strict quality control measures, this kit assures robust performance and simple usability, making it an excellent tool for researchers exploring the role of high-sensitivity CRP in inflammatory responses and associated health disorders.
Product Name:
Rat CRP ELISA Kit (High Sensitivity)
SKU:
AEES00518
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
