Rat Growth Hormone (GH) ELISA Kit (High Sensitivity)
The Rat HS GH (High Sensitive Growth Hormone) AccQuanti ELISA Kit is a powerful tool designed for the precise measurement of growth hormone levels in rat samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this ELISA kit ensures accurate and consistent results, making it suitable for a wide range of research applications. Growth hormone is a pivotal hormone that regulates growth, metabolism, and immune function in the body. Understanding its levels and activity is crucial for investigating various physiological processes and diseases, including growth disorders, metabolic disorders, and hormonal imbalances.
By using the Rat HS GH AccQuanti ELISA Kit, researchers can uncover valuable insights into growth hormone dynamics and its impact on health and disease. This kit provides a reliable and efficient method for analyzing growth hormone levels, enabling researchers to advance their understanding of growth regulation and potentially develop new therapeutic strategies.
Product Name:
Rat Growth Hormone (GH) ELISA Kit (High Sensitivity)
SKU:
AEFI00966
Reactivity:
Rat
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
1.875 pg/mL
Range:
3.125-200 pg/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
88-104%
86-92%
82-97%
EDTA Plasma (n = 5)
92-105%
82-100%
87-100%
Heparin Plasma (n = 5)
86-105%
88-98%
80-99%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
85-100
91
EDTA Plasma (n = 5)
87-105
99
Heparin Plasma (n = 5)
87-105
94
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
