The Rat IGF-1 (Insulin-like Growth Factor 1) CLIA Kit is a highly sensitive and specific assay designed for the accurate quantification of IGF-1 levels in rat serum, plasma, and tissue homogenates. This kit provides reliable and reproducible results, making it a valuable tool for a wide range of research applications.IGF-1 is a key regulator of growth and development, playing a crucial role in cell proliferation, differentiation, and survival.
Dysregulation of IGF-1 signaling has been implicated in various diseases, including cancer, diabetes, and aging-related conditions. The Rat IGF-1 CLIA Kit offers researchers a precise and effective means of studying the role of IGF-1 in these conditions and exploring potential therapeutic strategies.
Product Name:
Rat IGF-1 (Insulin-Like Growth Factor 1) CLIA Kit
SKU:
RTES00010
Target:
Rat IGF-1 (Insulin-Like Growth Factor 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IGF-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IGF-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IGF-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat IGF-1. You can calculate the concentration of Rat IGF-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-109
90-105
91-103
Average (%)
101
97
96
1:4
Range (%)
86-97
92-104
93-106
Average (%)
91
97
100
1:8
Range (%)
86-100
91-103
97-110
Average (%)
91
97
103
1:16
Range (%)
85-98
100-118
102-119
Average (%)
91
108
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-102
94
EDTA plasma (n=5)
93-107
99
Cell culture media (n=5)
97-109
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
39.76
95.95
349.43
42.96
98.56
338.71
Standard deviation
3.84
11.03
36.79
3.81
7.27
23.91
C V (%)
9.66
11.5
10.53
8.87
7.38
7.06
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat IGF-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat IGF-1 in samples. No significant cross-reactivity or interference between Rat IGF-1 and analogues was observed.
