The Rat IL-2 ELISA Kit is a specialized assay developed for the quantitative measurement of Interleukin-2 (IL-2) levels in diverse rat biological samples. IL-2 is a pivotal cytokine that plays a crucial role in immune regulation, particularly in the activation and proliferation of T cells. Its involvement in modulating immune responses and coordinating cellular interactions underscores its significance in various disease conditions, autoimmune disorders, and infections. Accurate detection of IL-2 levels is essential for unraveling the intricate mechanisms of immune function and for designing precise therapeutic interventions. Our IL-1 ELISA Kit is meticulously crafted to deliver superior sensitivity and specificity, ensuring precise and reproducible results. Manufactured under stringent quality control measures, this kit offers robust performance and user-friendly protocols, making it an indispensable tool for researchers aiming to investigate the functions and impact of IL-2 in immunological processes.
Product Name:
Rat IL-2 ELISA Kit
SKU:
AEES00295
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
