The Rat NOX1 (NADPH oxidase 1) ELISA Kit is a powerful tool for researchers looking to accurately measure NOX1 levels in rat samples. This kit is designed for use with rat serum, plasma, and cell culture supernatants, providing high sensitivity and specificity for reliable results in a variety of research applications. NOX1 is a key enzyme involved in the production of reactive oxygen species, playing a critical role in oxidative stress and inflammation. Abnormal NOX1 activity has been linked to a variety of diseases, including cardiovascular disorders, neurodegenerative conditions, and cancer.
This ELISA kit allows for the precise quantification of NOX1 levels, making it an invaluable tool for studying the role of this enzyme in disease pathology and developing potential therapeutic interventions. Overall, the Rat NOX1 ELISA Kit offers researchers a precise and efficient method for measuring NOX1 levels in rat samples, facilitating a deeper understanding of the role of this enzyme in health and disease.
Product Name:
Rat NOX1/NADPH oxidase 1 ELISA Kit
SKU:
RTFI00525
Reactivity:
Rat
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.188 ng/mL
Range:
0.313-20 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
85-104%
82-101%
80-100%
EDTA Plasma (n = 5)
86-99%
87-101%
90-100%
Heparin Plasma (n = 5)
89-103%
87-100%
80-94%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
86-104
95
EDTA Plasma (n = 5)
85-98
91
Heparin Plasma (n = 5)
89-105
96
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
