The Rat SOCS1 (Suppressor of Cytokine Signaling 1) ELISA Kit is a powerful tool for researchers looking to accurately measure levels of SOCS1 in rat samples. This kit is specifically designed for use with rat serum, plasma, and cell culture supernatants, providing a reliable and reproducible method for detecting this important protein. SOCS1 is known for its role in regulating the immune response and inflammation, making it a key player in various disease processes. By accurately measuring SOCS1 levels, researchers can gain valuable insights into the mechanisms underlying conditions such as autoimmune diseases, infectious diseases, and cancer.
With high sensitivity and specificity, the Rat SOCS1 ELISA Kit offers researchers confidence in their results, allowing for precise quantification of SOCS1 levels in rat samples. This kit is an essential tool for studies investigating the role of SOCS1 in health and disease, offering a comprehensive solution for measuring this critical protein.
Product Name:
Rat SOCS1 ELISA Kit
SKU:
RTFI00382
Reactivity:
Rat
Assay Type:
Sandwich ELISA, Double Antibody
Sensitivity:
0.188 ng/mL
Range:
0.313-20 ng/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C for 12 months.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 5)
91-97%
88-101%
86-103%
EDTA Plasma (n = 5)
87-98%
82-96%
86-101%
Heparin Plasma (n = 5)
82-99%
83-98%
84-94%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 5)
86-101
94
EDTA Plasma (n = 5)
85-103
93
Heparin Plasma (n = 5)
85-102
94
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2
Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3
Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4
HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5
Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6
Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately.
Plasma
Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant.
Cell Culture Supernatant
Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant.
Cell Lysate
Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant.
Other Sample Types
For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
