The Rat TF (Tissue Factor) ELISA Kit is specifically designed for the precise measurement of tissue factor levels in rat serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing dependable and consistent results, suitable for various research purposes.Tissue factor is a key protein involved in blood clotting and plays a critical role in regulating hemostasis. Dysregulation of tissue factor can lead to thrombotic disorders, cardiovascular diseases, and other pathological conditions. Accurate measurement of tissue factor levels is essential for understanding the underlying mechanisms of these diseases and developing novel therapeutic interventions.
Product Name:
Rat TF (Tissue Factor) ELISA Kit
SKU:
RTES00823
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
