The Rat TG (Thyroglobulin) CLIA Kit is a highly accurate assay designed for the quantitative measurement of thyroglobulin levels in rat serum, plasma, and tissue samples. This kit offers exceptional sensitivity and specificity, providing precise and consistent results for a variety of research applications.Thyroglobulin is a key protein involved in thyroid hormone production and is an important marker for thyroid function and disease. Monitoring thyroglobulin levels can help in the diagnosis and management of thyroid disorders such as cancer, hyperthyroidism, and hypothyroidism.
With its reliable performance and ease of use, the Rat TG CLIA Kit is essential for researchers studying thyroid function and diseases in rat models. By accurately measuring thyroglobulin levels, this kit plays a crucial role in advancing our understanding of thyroid biology and developing potential treatments for thyroid-related conditions.
Product Name:
Rat TG (Thyroglobulin) CLIA Kit
SKU:
RTES00552
Target:
Rat TG (Thyroglobulin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
1.88 pg/mL
Detection range:
3.13-200 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TG. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TG, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat TG. You can calculate the concentration of Rat TG in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-111
99-114
86-98
Average (%)
105
107
93
1:4
Range (%)
102-118
87-101
93-105
Average (%)
109
94
100
1:8
Range (%)
100-117
98-114
93-108
Average (%)
108
106
101
1:16
Range (%)
91-105
94-108
96-110
Average (%)
99
101
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-115
107
EDTA plasma (n=5)
90-103
95
Cell culture media (n=5)
102-117
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
10.04
31.57
79.38
10.38
29.35
84.37
Standard deviation
1.2
3.22
7.48
1.0
3.4
6.18
C V (%)
11.95
10.2
9.42
9.63
11.58
7.32
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Rat TG concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Rat TG in samples. No significant cross-reactivity or interference between Rat TG and analogues was observed.
