The Recombinant ALOX15 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C341055) against ALOX15. This gene encodes a member of the lipoxygenase family of proteins. The encoded enzyme acts on various polyunsaturated fatty acid substrates to generate various bioactive lipid mediators such as eicosanoids, hepoxilins, lipoxins, and other molecules. The encoded enzyme and its reaction products have been shown to regulate inflammation and immunity. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human and African green monkey samples.
This antibody is suitable for IHC, IF and FCM applications. It has been validated by IHC analysis of Human endometrial cancer, Human endometrium, IF analysis of HeLa, COS-7 and FCM analysis of HeLa. The target is localized to the membrane, cytoplasm, lipid droplet.
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Flow cytometric analysis of human ALOX15 expression on HeLa cells. Cells were stained with purified anti- Human ALOX15, then a Alexa Fluor 488-conjugated second step antibody. The histogram were derived from events with the forward and side light-scatter characteristics of intact cells.
Immunofluorescent analysis of (4% Paraformaldehyde) fixed HeLa cells using anti-ALOX15 Monoclonal Antibody at dilution of 1:50.
Immunofluorescent analysis of (4% Paraformaldehyde) fixed COS-7 cells using anti-ALOX15 Monoclonal Antibody at dilution of 1:50.
Immunohistochemistry of paraffin-embedded Human endometrial cancer using ALOX15 Monoclonal Antibody at dilution of 1:100.
Immunohistochemistry of paraffin-embedded Human endometrium using ALOX15 Monoclonal Antibody at dilution of 1:100.