The Recombinant Carbonic Anhydrase 9 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110677) against Carbonic Anhydrase 9. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IHC, IF, IP and ELISA applications. It has been validated by WB analysis of PC-12 and IHC analysis of Human colon carcinoma tissue. The target is localized to the membranous.
IHC 1:200-1000; WB 1:1000-5000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200
UniProt:
Q16790
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Immunohistochemistry of paraffin-embedded human colon carcinoma tissue using Recombinant Carbonic Anhydrase 9 Monoclonal Antibody at dilution of 1:200.
Western Blot with Recombinant Carbonic Anhydrase 9 Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: PC-12 cells. Observed-MW:49 kDa Calculated-MW:49 kDa