The Recombinant EN1/Engrailed 1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C431672) against EN1/Engrailed 1. Engrailed 1(EN1) is required for proper formation of the apical ectodermal ridge and correct dorsal-ventral patterning in the limb. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Mouse and Rat samples.
This antibody is suitable for WB applications. It has been validated by WB analysis of Mouse brain,?Rat brain. The target is localized to the nucleus.
Product Name:
Recombinant EN1/Engrailed 1 Monoclonal Antibody
SKU:
RMEA1672
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
EN1
Reactivity:
Mouse, Rat
Immunogen:
Peptide. This information is proprietary to PTMab.
Applications:
WB
Clonality:
Recombinant Monoclonal
Clone No:
C431672
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
EN1/Engrailed 1
Conjugation:
Unconjugated
Observed MW:
53 kDa
Calculated MW:
40 kDa
Recommended Dilution:
WB 1:1000
UniProt:
Q05925
Purification:
Protein A
Concentration:
1 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with EN1/Engrailed 1 Monoclonal Antibody at dilution of 1:1000. Lane 1: Mouse brain, Lane 2: Rat brain Observed-MW:53 kDa Calculated-MW:40 kDa