FGF acidic (ECGF/FGF-1/HBGF-1) is a non-glycosylated heparin binding growth factor that may be found in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes, and endothelial cells. It's a mitogenic protein that is made by many cell types, including mesodermal, ectodermal, and endodermal cells, and promotes the growth of cells from these three layers. FGF receptors must be activated in the presence of heparan sulfate. In the end, FGF acidic is transported to the nucleus, where it is phosphorylated by nuclear PKC delta and then exported to the cytosol and degraded. Intracellular FGF acidic inhibits p53 function and proapoptotic signaling. Human FGF/FGFa Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 0.2-2 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Fibroblast growth factor 1/Fibroblast Growth Factor Acidic is produced by our E.coli expression system and the target gene encoding Phe16-Asp155 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
15.9 kDa
AP Mol Mass:
16 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM Tris; 400mM NaCl; 1mM DTT; pH 8.0 .
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
