Interleukin-22(IL-22) is a member of the IL-10 family, a group of potent inflammatory mediators. IL-22 is produced by activated DC and T cells. The IL-22 and IL-10 receptors are involved in cell targeting and signal transduction. It activates and controls innate immune responses against bacterial infections, particularly in epithelial cells like respiratory and gut epithelial cells. IL-22, along with IL-17, is involved in the coordinated response of both adaptive and innate immune systems. IL-22 has the ability to promote hepatocyte survival in the liver and epithelial cells in the lung and gut, which is similar to IL-10. The biological activity of IL-22 is activated by binding to a cell-surface complex made up of the IL-22R1 and IL-10R2 receptor chains. The biological activity of IL-22 is also controlled by interactions with a soluble binding protein, called IL-22BP. IL-22BP and an extracellular region of IL-22R1 share sequence similarity. In some situations, IL-22's pro-inflammatory vs. tissue-protective activities are regulated by cytokine IL-17A. Human Interleukin-22/IL-22 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Product Name:
Human Interleukin-22/IL-22 Recombinant Protein (RPES1532)
Measured by its ability to induce IL-10 secretion in COLO 205 human colorectal adenocarcinoma cells. The ED50 for this effect is 0.11 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Interleukin-22 is produced by our E.coli expression system and the target gene encoding Ala34-Ile179 is expressed.
Purity:
> 95% as determined by reducing SDS-PAGE.
Mol Mass:
16.9 kDa
AP Mol Mass:
14 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM PB; 150mM NaCl; pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
