There exists two types of tryptophanyl tRNA synthetases, the cytoplasmic form called WARS, the mitochondrial form called WARS2. WARS catalyzes the aminoacylation of tRNA (trp) with tryptophan and is induced by interferon. WARS regulates ERK, Akt, eNOS activation pathway, which are related with angiogenesis, cytoskelatal reorganization and shear stess-reponsive gene expression.
Product Name:
Human WARS/TrpRS Recombinant Protein (RPES1526)
Product Code:
RPES1526
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
WARS also known as Tryptophanyl-tRNA synthetase, Interferon-induced protein 53.
Accession:
P23381
Sequence:
Met 1-Gln471
Fusion tag:
N-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Tryptophan--tRNA ligase, cytoplasmic is produced by our E.coli expression system and the target gene encoding Met1-Gln471 is expressed with a 6His tag at the N-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
55.3 kDa
AP Mol Mass:
60 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 20mM Tris, 150mM NaCl, pH 8.0.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
