The Recombinant LATS1 Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C170415) against LATS1. The protein encoded by this gene is a putative serine/threonine kinase that localizes to the mitotic apparatus and complexes with cell cycle controller CDC2 kinase in early mitosis. The protein is phosphorylated in a cell-cycle dependent manner, with late prophase phosphorylation remaining through metaphase. The N-terminal region of the protein binds CDC2 to form a complex showing reduced H1 histone kinase activity, indicating a role as a negative regulator of CDC2/cyclin A. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.
This antibody is suitable for WB, IF and ELISA applications. It has been validated by WB analysis of C2C12. The target is localized to the cytoplasm.
Product Name:
Recombinant LATS1 Monoclonal Antibody
SKU:
RMEA0428
Size:
50 µL, 100 µL
Antibody Type:
Recombinant Monoclonal Antibody
Synonyms:
LATS, LATS1, WARTS, wts
Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant Human LATS1 protein
Applications:
WBIFELISA
Clonality:
Recombinant Monoclonal
Clone No:
C170415
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
LATS1
Conjugation:
Unconjugated
Observed MW:
140 kDa
Calculated MW:
127 kDa
Recommended Dilution:
WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000
UniProt:
O95835
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with Recombinant LATS1 Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: C2C12 cell lysate. Observed-MW:140 kDa Calculated-MW:127 kDa
