The Recombinant Phospho-CREB-1 (Ser133) Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C110233) against CREB-1 (Ser133). This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse, Rat and Pig samples.
This antibody is suitable for WB, IHC, IF, IP and ELISA applications. It has been validated by WB analysis of Hela. The target is localized to the nucleus.
Active transcription factor CREB, cAMP response element binding protein, cAMP response element binding protein 1, cAMP responsive element binding protein 1, cAMP-responsive element-binding protein 1, CREB, CREB-1, CREB1, Cyclic AMP-responsive element-binding protein 1, MGC9284, OTTHUMP00000163864, OTTHUMP00000163865, OTTHUMP00000206660, OTTHUMP00000206662, OTTHUMP00000206667, Transactivator protein
Reactivity:
Human, Mouse, Rat, Pig
Immunogen:
A synthetic peptide corresponding to residues around (Ser133) of Human Phospho-CREB-1
Applications:
WBIHCIFIPELISA
Clonality:
Recombinant Monoclonal
Clone No:
C110233
Host:
Rabbit
Isotype:
Rabbit IgG
Target:
CREB-1 (Ser133)
Conjugation:
Unconjugated
Observed MW:
43 kDa
Calculated MW:
37 kDa
Recommended Dilution:
IHC 1:1000-4000; WB 1:2000-10000; IF 1:200-1000; ELISA 1:5000-20000; IP 1:50-200
UniProt:
P16220
Purification:
Protein A
Concentration:
0.2 mg/mL
Buffer:
PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:
Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
Western Blot with Recombinant Phospho-CREB-1 (Ser133) Monoclonal Antibody at dilution of 1:1000 dilution. Lane A: Hela cell lysate. Observed-MW:43 kDa Calculated-MW:37 kDa