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Recombinant Phospho-IKB alpha (Ser32, Ser36) Monoclonal Antibody (RMEA1704)

Datasheet MSDS
SKU RMEA1704
Product Type Antibody
Reactivity Human, Rat, Mouse
Applications WB, IHC
Host Species Rabbit
Clonality Monoclonal
Clone C121704
Conjugate Unconjugated
€459 - €639

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Description

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Recombinant Phospho-IKB alpha (Ser32, Ser36) Monoclonal Antibody

The Recombinant Phospho-IKB alpha (Ser32, Ser36) Monoclonal Antibody is a recombinant rabbit monoclonal antibody (clone C121704) against IKB alpha (Ser32, Ser36). NF-kappa-B inhibitor alpha (IKB alpha) inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, IKB alpha becomes phosphorylated promoting itself ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. Produced by recombinant technology in Rabbit hosts and purified by Protein A affinity chromatography, this Rabbit IgG antibody is reactive against Human, Mouse and Rat samples.

This antibody is suitable for WB and IHC applications. It has been validated by WB analysis of HeLa, C6 and IHC analysis of (A) Rat kidney, (B) Rat kidney, then the tissue was incubated with alkaline phosphatase (50U/ml) at 37°C for 1 hour, (A) Mouse kidney, (B) Mouse kidney, then the tissue was incubated with alkalinephosphatase (50 U/ml) at 37°C for 1 hour. The target is localized to the nucleus, cytoplasm.

Product Name:Recombinant Phospho-IKB alpha (Ser32, Ser36) Monoclonal Antibody
SKU:RMEA1704
Size:50 µL, 100 µL
Antibody Type:Recombinant Monoclonal Antibody
Synonyms:alpha, EDAID, EDAID2, I kappa B alpha, I-kappa-B-alpha, IkappaBalpha, IKB alpha, IkB-alpha, IKBA, IKBalpha, MAD, MAD 3, MAD-3, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF kappa B inhibitor alpha, NF-kappa-B inhibitor alpha, NFKBI, NFKBIA, Nuclear factor of kappa light chain gene enhancer in B cells, Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, pIκBα
Reactivity:Human, Mouse, Rat
Immunogen:Recombinant human IKB alpha (phospho S32/36) fragment
Applications:WBIHC
Clonality:Recombinant Monoclonal
Clone No:C121704
Host:Rabbit
Isotype:Rabbit IgG
Target:IKB alpha (Ser32, Ser36)
Conjugation:Unconjugated
Observed MW:36 kDa
Calculated MW:36 kDa
Recommended Dilution:WB 1:500-2000; IHC 1:50-100
UniProt:P25963
Purification:Protein A
Concentration:1 mg/mL
Buffer:PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Storage:Store at -20°C valid for 12 months. Avoid freeze / thaw cycles.
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