Description
SARS-CoV-2 Spike (S) S1 Recombinant Protein
The SARS-CoV-2 Spike (S) S1 Recombinant Protein represents a critical molecular component of the SARS-CoV-2 virus, playing an essential role in viral entry and host cell infection mechanisms. This protein is extensively utilized in COVID-19 research, vaccine development, therapeutic antibody screening, and diagnostic assay development, serving as a fundamental tool for understanding viral pathogenesis and developing countermeasures against coronavirus infections.
This product (SKU: CARP01262) is produced using HEK293 cells and features a C-His tag for convenient detection and purification. The protein exhibits a calculated molecular weight of 75.85 kDa with an observed molecular weight of 110-130 kDa under denaturing conditions, achieving ≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC.. Functional bioactivity has been validated through rigorous quality control assays, confirming its suitability for demanding research applications.
Key Features
High Purity by Affinity Chromatography | |
Mammalian & Bacterial Expression Systems | |
High lot-to-lot consistency via strict QC |
Product Name: | SARS-CoV-2 Spike (S) S1 Recombinant Protein |
SKU: | CARP01262 |
Size: | 100 μg |
Reactivity: | SARS-CoV-2 |
Synonyms: | Envelope, SARS-CoV-2 Spike RBD (N501Y), Spike, Spike ECD, Spike RBD, Spike S1, Spike S2, Spike S2 ECD, S1-RBD protein, NCP-CoV RBD Protein, novel coronavirus RBD Protein, 2019-nCoV RBD Protein, S glycoprotein Subunit1 RBD Protein |
Tag: | C-His |
Expression Host: | HEK293 cells |
Calculated MW: | 75.85 kDa |
Observed MW: | 110-130 kDa |
Gene ID: | 43740568 |
Protein Description: | High quality, high purity and low endotoxin recombinant Recombinant SARS-CoV-2 Spike S1 Protein (CARP01262), tested reactivity in HEK293 cells and has been validated in SDS-PAGE.100% guaranteed. |
Endotoxin: | < 1 EU/μg of the protein by LAL method. |
Purity: | ≥ 95 % as determined by SDS-PAGE;≥ 95 % as determined by HPLC. |
Formulation: | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. or Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.Contact us for customized product form or formulation. |
Bio-Activity: |
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Reconstitution: | Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles. |
Storage: | Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week. |
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates thisinteraction.The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.